Addgene

We narrowed to 13,076 results for: FLU

Showing: 641 - 660 of 13076 results

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csr-1_son-1_mRuby3

Plasmid

#191744

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mRuby3

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mNeptune2.5

Plasmid

#191748

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Use

Tags

NCU04502 promoter and mNeptune2.5

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LLP722_pSP15

Plasmid

#192435

Purpose

To test the relative expression strength of the TCTP1 promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

TCTP1::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP720_pSP13

Plasmid

#192433

Purpose

To test the relative expression strength of the 35S promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

35S::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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LLP721_pSP14

Plasmid

#192434

Purpose

To test the relative expression strength of the Act2 promoter

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Mutation

Promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf

Plasmid

#182000

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_STf_SKL

Plasmid

#182007

Purpose

CMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell lines

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

STf_SKL

Use

Tags

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEGFP

Plasmid

#190748

Purpose

Expression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2 and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)

Plasmid

#190746

Purpose

Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)-mEGFP

Plasmid

#190750

Purpose

Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEGFP and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182854

Purpose

Hetero-tetrmer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mCherry2-mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Use

Tags

Histag and SpyTag

Expression

Bacterial

Mutation

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pfMDH_K381A

Plasmid

#67732

Purpose

Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed lysine 381 to alanine

Promoter

T7

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_N300D

Plasmid

#67728

Purpose

Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 300 to aspartate

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_R373A

Plasmid

#67731

Purpose

Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed arginine 373 to alanine

Promoter

T7

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pfMDH_N300S

Plasmid

#67729

Purpose

Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Use

Tags

6x-His tag

Expression

Bacterial

Mutation

Changed asparagine 300 to serine

Promoter

T7

Available since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
mCerulean-Zyxin-6

Plasmid

#55398

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Zyxin (ZYX Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-Tubulin-6

Plasmid

#56450

Purpose

Localization: Microtubules, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Tubulin (TUBA1B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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