We narrowed to 7,122 results for: ef1a

1. Lenti-EF1alpha-dCas9-KRAB_Hygro

   Plasmid

   #192663

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-GFP ArgSen

   Plasmid

   #184704

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. CSII-prEF1a-mCherry-3xNLS

   Plasmid

   #125262

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   mCherry-3xNLS
   

   Use

   Lentiviral

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lv EF1a MS2-Fkbp2x 2A Hygro

   Plasmid

   #102807

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   MS2-Fkbp2x
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLv-EF1a-Parkin-A92mKO2

   Plasmid

   #213559

   Purpose

   Lentiviral vector for stable expression of Parkin, internally tagged with mKO2

   Depositor

   Lani Wu, Steven Altschuler

   Article

   Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

   Insert

   Parkin (PRKN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

   Plasmid

   #89150

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM4D(Gi)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX-EF1a_NKX2-1_Short_V5-GFP

   Plasmid

   #221497

   Purpose

   NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Tags

   V5-linker-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-fDIO-GCaMP6f

   Plasmid

   #128315

   Purpose

   Expresses GCaMP6f under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   His Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAID1.2-EF1a-NmScarlet-mAID

   Plasmid

   #140617

   Purpose

   Expression of OsTIR1 and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAID1.2-EF1a-CmCherry-mAID

    Plasmid

    #140604

    Purpose

    Expression of OsTIR1 and mAID-mCherry protein in mammalian cells under the control of EF1a promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-EF1aL-WT_ABCA3-GFP

    Plasmid

    #188548

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1aL

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT3-EF1aH-TAZ S89A

    Plasmid

    #179910

    Purpose

    Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Use

    Tags

    3X Flag-tag

    Expression

    Mammalian

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    EF1a

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CSII-prEF1a-E2-Crimson-NLS

    Plasmid

    #125263

    Purpose

    Fluorescent reporter for cell size

    Depositor

    Jan Skotheim

    Article

    Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

    Insert

    E2-Crimson-NLS
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-EF1a-jGCaMP8f-WPRE

    Plasmid

    #176756

    Purpose

    AAV-mediated expression of GCaMP8f under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-EF1a-MegaGate-HA-Hygro

    Plasmid

    #175268

    Purpose

    MegaGate destination with C-terminal HA tag and hygromycin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DO-EGFP-WPRE-pA

    Plasmid

    #37085

    Purpose

    Expresses EGFP in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-EF1a-MegaGate-HA-Blast

    Plasmid

    #175270

    Purpose

    MegaGate destination with C-terminal HA tag and blasticidin resistance

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRRLNeo-pEF1a-p53ash-mCerulean

    Plasmid

    #69579

    Purpose

    Expresses p53 tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Hygro-EF1A-LivePARBackbone

    Plasmid

    #176526

    Purpose

    Expression vector with a BamHI site in-frame with a Gly-Ser linker fused to EGFP; serves as the backbone for PAR binding domain incorporation for LivePAR

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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