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Plasmid#15152DepositorInsertEngrailed repressor (en Fly)
UseRetroviralTagsExpressionMammalianMutationengrailed repressor domainPromoterAvailable sinceJune 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromotertet-on and CBhAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCDHGC5 sh1 shRNA
Plasmid#122227PurposeKnock Down Protocadherin gamma C5. It targets Protocadherin gamma C5 mRNA (nucleotides 851–871 in the protein coding region).DepositorInsertPCDHGC5 shRNA (Pcdhgc5 Rat)
UseRNAiTagsExpressionMutationPromoterAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-Avr-HPRT-RIGHT
Plasmid#107272PurposeExpresses TALEN (right) targeting HPRT exon3DepositorInsertHPRT1_B Avr-TALEN (right)
UseTALENTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-Avr-HPRT-LEFT
Plasmid#107271PurposeExpresses TALEN (left) targeting HPRT exon3DepositorInsertHPRT1_B Avr-TALEN (left)
UseTALENTags3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin GFP
Plasmid#72919Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving GFPDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gg 3kb Green opsin DsRed
Plasmid#72918Purposegreen opsin promoter from chicken (Gallus gallus) gDNA chr26:4,504,913–4,501,931 in galGal4 driving DsRedDepositorInsertchicken green opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
mito-PAGFP
Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
UseTagsmitoExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-AF8
Plasmid#117803Purposemammalian expression of GFP-tagged human centromere protein ADepositorInsertCENP-A (CENPA Human)
UseTagsGFPExpressionMammalianMutationSee depositor comments below.PromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 WT
Plasmid#198201PurposeFRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenusDepositorInsertMYO5A IQ domain (Myo5a Mouse)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330_DRS-1 sgRNA / hSpCas9
Plasmid#172841PurposeMammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertDRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SaCas9
Plasmid#178901PurposeSaCas9 (Cas9 from Staphylococcus aureus)DepositorInsertSaCas9
UseTagsHis6-Tag, bipartite nuclear localization signal f…ExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga2
Plasmid#122294PurposeOverexpression for mouse Hmga2DepositorInsertHmga2 (Hmga2 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179120PurposeExpresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertcontrol sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179119PurposeExpresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertEzr sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-synPCB2.1
Plasmid#139478PurposeExpression vector for phycocyanobilin (PCB) synthesis in mammalian cells.DepositorInsertMTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG2IG-Hmga1a
Plasmid#122293PurposeOverexpression for mouse Hmga1aDepositorInsertHmga1a (Hmga1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179117PurposeExpresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertFermt2 sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only