We narrowed to 9,982 results for: otos

1. pET-21(+)Tm9D8* TrpB

   Plasmid

   #207517

   Purpose

   Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   Tryptophan synthase β-subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Dronpa-Lifeact-7

   Plasmid

   #54740

   Purpose

   Localization: Actin, Excitation: 503, Emission: 518

   Depositor

   Michael Davidson

   Article

   Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

   Type

   Empty backbone

   Use

   Tags

   Dronpa-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pECFP-histone H3

   Plasmid

   #179698

   Purpose

   visualization of nucleus/chromosome

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Histone H3 (H3c10 Mouse)
   

   Use

   Tags

   ECFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A206K both

   Promoter

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-Lifeact_Halo_T2A_EGFP

   Plasmid

   #135445

   Purpose

   Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   Lifact-HaloTag7 and EGFP
   

   Use

   Tags

   Lifact

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCSIIhyg-rtTA

   Plasmid

   #139480

   Purpose

   Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   rtTA, Tet-ON reverse transactivator
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human EF1a promoter

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pT2ADWpuro_2paRAF

   Plasmid

   #129653

   Purpose

   For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBAD-T-GECO1

   Plasmid

   #191616

   Purpose

   Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

   Depositor

   Robert Campbell

   Article

   Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

   Insert

   T-GECO1
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pT3-Neo

    Plasmid

    #69123

    Purpose

    Sleeping Beauty (SB) transposon with G418 resistance

    Depositor

    Martin Bonamino

    Article

    Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

    Insert

    Neo (codon optimized for human)
    

    Use

    Sleeping beauty transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U3MSCV

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE80l A-CC-A

    Plasmid

    #196478

    Purpose

    Protein expression of SpyTag-ELP-CarHCC-ELP SpyTag, with GRGD site linked with (GGGGS)2 before CarHCC and after first ELP

    Depositor

    Fei Sun

    Article

    Yang et al Sci Adv. 2022 Apr;8(13):eabm5482. doi: 1

    Insert

    A-CC-A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS CRX (CC#4)

    Plasmid

    #15127

    Depositor

    Connie Cepko

    Article

    Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

    Insert

    CRX (Crx Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 8, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pENTR-mOvol1

    Plasmid

    #120359

    Purpose

    Gateway entry vector containing mouse Ovol1.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT2ADW_2paRAF

    Plasmid

    #129654

    Purpose

    For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. sspa-GCaMP6s

    Plasmid

    #67559

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRSET-triple mutation-pa-GCaMP6f

    Plasmid

    #67570

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    V115H, L64F, T65S

    Promoter

    T7

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. NK-1 GFP

    Plasmid

    #24508

    Depositor

    Ron Vale

    Article

    Case et al Cell. 1997 Sep 5. 90(5):959-66.

    Insert

    Drosophila NCD, human kinesin
    

    Use

    Tags

    GFP and His

    Expression

    Bacterial

    Mutation

    NK-1 is a chimera where the entire superfamily-co…

    Promoter

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLIA CRX (CC#69)

    Plasmid

    #15128

    Depositor

    Connie Cepko

    Article

    Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

    Insert

    CRX (Crx Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only