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HA-Rab8a-CA (Q67L)

Plasmid

#101050

Purpose

Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.15_pSOX17

Plasmid

#195496

Purpose

Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Promoter region of SOX17 (SOX17 Human)

Use

Tags

luc2-hPEST

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-MED1trunc-IDR-YFP-NLS

Plasmid

#145275

Purpose

Mammalian Expression of Nuclear MED1 truncated IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR truncated (MED1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13(+10A)-IDR-mCherry

Plasmid

#145282

Purpose

Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+10A)-IDR (HOXD13 Human)

Use

Tags

Expression

Bacterial

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-WT

Plasmid

#215601

Purpose

For overexpression of Gal4-DBD CEBPa WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13-IDR-mCherry-CRY2-NLS

Plasmid

#145260

Purpose

Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4.27_eSOX17

Plasmid

#195498

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Full distal endoderm enhancer region of SOX17 (SOX17 Human)

Use

Tags

minP_luc2-hPEST

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.27_eSOX17.2

Plasmid

#195499

Purpose

Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17

Depositor

Alexander Meissner

Article

Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

Insert

Core distal endoderm enhancer region of SOX17 (SOX17 Human)

Use

Tags

minP_luc2-hPEST

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-TetON-mEGFP-NGN2_WT

Plasmid

#215610

Purpose

For integration of NGN2 WT T2A mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Use

Tags

T2A mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

Plasmid

#145263

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+9A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

Plasmid

#117319

Purpose

Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-CTRL

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Mutation

miR-124 deleted

Promoter

Tet-inducible promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-WT

Plasmid

#215592

Purpose

For overexpression of Gal4-DBD EGR1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 (EGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXB1-WT

Plasmid

#215589

Purpose

For overexpression of Gal4-DBD HOXB1 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXB1 (HOXB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa_WT(N)

Plasmid

#215635

Purpose

For overexpression of Gal4-DBD CEBPa WT(N)

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa(N) (CEBPA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EGR1-AroSCRAMBLED

Plasmid

#215594

Purpose

For overexpression of Gal4-DBD EGR1 AroSCRAMBLED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

EGR1 AroSCRAMBLED (EGR1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS_PATCHED

Plasmid

#215584

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS PATCHED

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS PATCHED (HOXD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-CEBPa-AroPERFECT_IS15+2

Plasmid

#215605

Purpose

For overexpression of Gal4-DBD CEBPa AroPERFECT IS15+2

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15+2 (CEBPA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD4-AroPLUS

Plasmid

#215582

Purpose

For overexpression of Gal4-DBD HOXD4 AroPLUS

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXD4 AroPLUS (HOXD4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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