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pDONR207 SARS-CoV-2 NSP3

Plasmid

#141257

Purpose

Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Has Service

Cloning Grade DNA

Insert

NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PAK3

Plasmid

#23439

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK3 (PAK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC25A51

Plasmid

#132019

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A51 (SLC25A51 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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MIGR1-Cdc42-1

Plasmid

#179517

Purpose

Retroviral expression of Cdc42 splice variant 1

Depositor

Bo Porse

Article

Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

Insert

Cdc42 splice variant 1 cDNA (CDC42 Human)

Use

Retroviral

Tags

IRES-EGFP

Promoter

LTR

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDK4

Plasmid

#23577

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK4 (PDK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
MIGR1-Cdc42-2

Plasmid

#179518

Purpose

Retroviral expression of Cdc42 splice variant 2

Depositor

Bo Porse

Article

Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

Insert

Cdc42 splice variant 2 cDNA (CDC42 Human)

Use

Retroviral

Tags

IRES-EGFP

Promoter

LTR

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pMx-MGT

Plasmid

#111810

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

viral LTR

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZAP70

Plasmid

#23887

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ZAP70 (ZAP70 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-NTRK2

Plasmid

#23883

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK2 (NTRK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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Lenti-TetON-Sox2

Plasmid

#110280

Purpose

Doxycycline-inducible overexpression of Sox2

Depositor

Trudy Oliver

Article

Mollaoglu et al Immunity. 2018 Oct 16;49(4):764-779.e9.

Insert

SRY (sex determining region Y)-box 2 (Sox2 Mouse)

Use

Lentiviral

Tags

No fusion genes

Expression

Mammalian

Mutation

Wild type

Promoter

TRE

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-SYK

Plasmid

#23907

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SYK (SYK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME6

Plasmid

#23562

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME6 (NME6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221_MFSD8

Plasmid

#131882

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD8 (MFSD8 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223-PRKAG3

Plasmid

#23549

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAG3 (PRKAG3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PGK1

Plasmid

#23427

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PGK1 (PGK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLKO.1.sh.beta-catenin.1248

Plasmid

#19761

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

small hairpin RNA against beta-catenin (CTNNB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-ARNT

Plasmid

#203578

Purpose

Expresses wild-type ARNT in mammalian cells.

Depositor

Abhishek Chakraborty, William Kaelin

Article

Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

Insert

ARNT (ARNT Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-CCNE1

Plasmid

#162553

Purpose

Overexpression of CCNE1

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE1 (CCNE1 Human)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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P527_POU4F2-p2A-mNeonGreen-h2b

Plasmid

#239103

Purpose

Azenta NGS; inserts a p2A-mNeonGreen-h2b fusion at the endogenous POU4F2 (aka BRN3B) gene

Depositor

Karl Wahlin

Article

Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

Insert

POU4F2 (POU4F2 Human)

Tags

p2A-mNeonGreen-h2b

Expression

Mammalian

Promoter

Endogenous POU4F2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#129418

Purpose

Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgRAB18.N (RAB18 Human)

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-TLK2

Plasmid

#23629

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TLK2 (TLK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KA5

Plasmid

#23758

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA5 (RPS6KA5 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMXs-HRAS(G12V)-IRES-SNAP-p53DD

Plasmid

#233200

Purpose

Retroviral expression of HRAS(G12V) and SNAP-p53DD

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

HRAS(G12V)-IRES-SNAP-p53DD (Trp53, HRAS Human, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pMXs-SNAP-p53DD-IRES-HRAS(G12V)

Plasmid

#233198

Purpose

Retroviral expression of SNAP-p53DD and HRAS(G12V)

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

Insert

SNAP-p53DD-IRES-HRAS(G12V) (HRAS, Trp53 Human, Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223 SARS-CoV-2 NSP12

Plasmid

#141265

Purpose

Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221-MFSD6_STOP

Plasmid

#161119

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD6 (MFSD6 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PTK2

Plasmid

#23902

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2 (PTK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT/TO-3*Flag-APEX2 N-term LMNA

Plasmid

#187576

Purpose

Express FLAG epitope and APEX2-tagged LMNA fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

LMNA (LMNA Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

Plasmid

#232343

Purpose

Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

Depositor

Philip Wong

Article

Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

Insert

HDGF like 2 (HDGFL2 Human)

Tags

GGGS linker, 6xHis and Twin-strep, TEV protease s…

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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