We narrowed to 22,937 results for: TES

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pEGFP-VCPIP1

Plasmid

#85671

Purpose

Expression of VCPIP1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

VCPIP1 (VCPIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-TEX264_wt-His6

Plasmid

#220214

Purpose

Bacterial expression of wild type TEX264, C-terminal His6 tag

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173873

Purpose

Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT6_H133Y

Plasmid

#53149

Purpose

expression vector for catalyitc inactive human Sirtuin6

Depositor

Katrin Chua

Article

Michishita et al Nature. 2008 Mar 27;452(7186):492-6. doi

Insert

Sirt6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H133Y, catalytic inactive

Promoter

CMV

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pBX_RPL7a_smRuby2_HA

Plasmid

#236087

Purpose

Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPL7a-smRuby2-HA (RPL7A Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(2)-CRY2-mCherry

Plasmid

#160441

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mycRSK2wt

Plasmid

#15827

Depositor

Jing Chen

Article

Kang et al Cancer Cell. 2007 Sep . 12(3):201-14.

Insert

Ribosomal S6 kinase 2 (Rps6ka3 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

wild type

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET19-MTdTwt

Plasmid

#207460

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT)

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTwt (Dntt Mouse)

Tags

10xHis-MBP

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-2A-ZsGreen

Plasmid

#231985

Purpose

Empty backbone for expression of GoI with Bi-cistronic expression ZsGreen

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

2A-ZsGreen

Use

Lentiviral

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-VPS35

Plasmid

#21689

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only
B2342_BTB-mCherry

Plasmid

#215095

Purpose

Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BCL6 BTB (BCL6 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Tags

Citrine

Expression

Plant

Promoter

ProRUBY

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIH-myc-hTAZ

Plasmid

#32841

Depositor

Kunliang Guan

Article

Lei et al Mol Cell Biol. 2008 Apr;28(7):2426-36. E

Insert

TAZ (WWTR1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV/MSV

Available Since

Nov. 15, 2011

Availability

Academic Institutions and Nonprofits only
hTPST1

Plasmid

#11252

Depositor

Hyeryun Choe

Article

Choe et al Cell. 2003 Jul 25. 114(2):161-70.

Insert

TPST1 (TPST1 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pFX-Pickles 2.34NES

Plasmid

#100028

Purpose

Improved FRET biosensor for the measurement of BCR-ABL activity.

Depositor

Yusuke Ohba

Article

Horiguchi et al Cell Struct Funct. 2017 Feb 2;42(1):15-2

Insert

Pickles-2.34NES

Use

Pfx vector is derived from pcmv vector.

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-CCSS

Plasmid

#83424

Purpose

Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residues 218 and 219 from C to S (CCSS: TGCTGT -&…

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 HA pCAGGS

Plasmid

#206054

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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NSP6-FR-2Strep

Plasmid

#210317

Purpose

Expresses SARS-CoV-2 NSP6 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

2Strep

Expression

Mammalian

Mutation

Many synonymous changes due to codon optimization

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 BARK1 minigene

Plasmid

#14695

Depositor

Robert Lefkowitz

Article

Koch et al J Biol Chem. 1994 Feb 25. 269(8):6193-7.

Insert

BARK1 minigene (GRK2 Bovine)

Tags

3'UTR of human b-globin

Expression

Mammalian

Mutation

BARK1 amino acids 495-689

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

Plasmid

#122083

Purpose

Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII-V5-LaminB1

Use

Retroviral

Tags

V5 tag on Nter of LaminB1

Expression

Bacterial and Mammalian

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-53BP1LCD

Plasmid

#204407

Purpose

E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

53BP1(1208-1977) (TP53BP1 Human)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mRFP-mem

Plasmid

#55622

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

Depositor

Catherine Berlot

Article

Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

Insert

mRFP-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

Plasmid

#47457

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-DN-REST

Plasmid

#61533

Purpose

Expresses DN-REST in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

REST (REST Human)

Use

Lentiviral

Expression

Mammalian

Mutation

P73, Dominant negative REST (contains the Zf DNA-…

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK2

Plasmid

#197110

Purpose

Expression of GFP-tagged TAOK2 / PSK1 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK2 (TAOK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Promoter

SFFV promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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