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px330-GAPDH

Plasmid

#136940

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human GAPDH; induce CD4+ deletion rearrangement by pairing w/ px330-CD4

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting GAPDH (GAPDH Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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px330-CD4

Plasmid

#136938

Purpose

NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

Depositor

Jeremy Stark

Article

Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

Insert

sgRNA targeting CD4 (CD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP-WPRE

Plasmid

#127864

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Promoter

hSyn

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Smpd4

Plasmid

#120237

Purpose

Expresses V5-tagged Smpd4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Smpd4 (Smpd4 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-RFP2

Plasmid

#106409

Purpose

P-RAN-RFP2 (RANbody RFP2::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-RFP2

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-Gelsolin

Plasmid

#106413

Purpose

P-RAN-Gelsolin (RANbody Gelsolin::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-Gelsolin

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-GFP

Plasmid

#105946

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC (Prkcz Rat)

Tags

CAAX and GFP

Expression

Bacterial

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_HOIP/RNF31_#6

Plasmid

#75252

Purpose

retroviral shRNA vector against human HOIP/RNF31, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against human RNF31 (RNF31 )

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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Ntn4-Fc-His

Plasmid

#72106

Purpose

Expresses the entire Netrin 4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn4 (Ntn4 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 DDX5

Plasmid

#67111

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

DDX5 (DDX5 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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mRFP-dmBACCS2

Plasmid

#67630

Purpose

Drosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

mRFP-dmBACCS2

Use

Drosophila expression

Promoter

Drosophila actin gene 5C gene promoter

Available Since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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RP156 pUAST EGFP:Gr21a

Plasmid

#59524

Purpose

N-terminal fusion of EGFP to Drosophila melanogaster Gr21a

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Gr21 (Gr21a Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pBS ColIII alpha1

Plasmid

#19187

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Procollagen type III alpha 1 (COL3A1 Chicken)

Expression

Bacterial

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf144

Plasmid

#12832

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf144

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
cBEST4

Plasmid

#234660

Purpose

Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

Depositor

Mingzi M. Zhang

Article

Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

Inserts

spCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3 and tcp830

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SV40NLSf-mScarlet-P2A-hLuc-WPRE

Plasmid

#203539

Purpose

AAV reporter genome plasmid

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

NLS-mScarlet-2A-mScarlet

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-myc

Plasmid

#218191

Purpose

Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

2xmyc and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pC1-dmito-oROS-HT

Plasmid

#216418

Purpose

Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to mitochondrial matrix.

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

dmito-oROS-HT

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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