We narrowed to 13,292 results for: cDNA

1. pcDNA3.1 His-GFP-EWSR1-myc-His

   Plasmid

   #46385

   Purpose

   expresses EGFP tagged EWSR1 in mammalian cells

   Depositor

   Heinz Gehring

   Article

   Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   EGFP, His, and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

2. (383) pcDNA3.1-HA-nBC2-OGA(544-706)

   Plasmid

   #168100

   Purpose

   HA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nBC2-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-EGFP MASTL G44S (siRNA resistant)

   Plasmid

   #191012

   Purpose

   Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

   Depositor

   Johanna Ivaska

   Article

   Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   Microtubule-associated serine/threonine kinase like (MASTL Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   G44S

   Promoter

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-Flag-H2A K13-15-118-119R

   Plasmid

   #63568

   Purpose

   Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine at 13, 15, 118, and 119 are now Arginine

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. CFP(159-238)-gamma-2 in pcDNAI/Amp

   Plasmid

   #55777

   Purpose

   A C-terminal CFP fragment was fused to Ggamma-2. When co-expressed with an N-terminal mCerulean, CFP, or YFP fragment fused to a Gbeta subunit with which it interacts a fluorescent signal is produced.

   Depositor

   Catherine Berlot

   Article

   Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

   Insert

   CFP(159-238)-gamma-2 (GNG2 Aequorea victoria, Human)
   

   Use

   Tags

   CFP(159-238) was fused to the amino terminus of G…

   Expression

   Mammalian

   Mutation

   CFP(159-238) includes a substitution of His for A…

   Promoter

   CMV

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-SREBP-1-GFP (A317-318; A341)

   Plasmid

   #204198

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   3xHA, eGFP and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317,318,341

   Promoter

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT/TO_COX8-CoA-Snifit(G41S)

   Plasmid

   #197943

   Purpose

   Expression of CoA-Snifit in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

   Insert

   Halo-GFP-ecPanK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PanK=PanK-G41S, D127A,L277W,F252Y

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

   Plasmid

   #100279

   Purpose

   In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

   Depositor

   Yi Zhang

   Article

   Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

   Insert

   Kdm6b (Kdm6b Mouse)
   

   Use

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   Mutated catalytic domain (1025aa-End) (please see…

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

   Plasmid

   #60814

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   eGFP-NLS (SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PEST

    Plasmid

    #127321

    Purpose

    Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG and CL1/PEST

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-IgKappa-myc-dL5- 2XG4S-GPI

    Plasmid

    #153308

    Purpose

    Expresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)

    Depositor

    Marcel Bruchez

    Article

    Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

    Insert

    IgKappa-myc-dL5-2XG4S-GPI
    

    Use

    Tags

    myc-dL5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 - PP1g - Flag - BirA* (AA2-140)

    Plasmid

    #86886

    Purpose

    N-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    PP1g - FLAG - BirA (AA2-140)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 2-140

    Promoter

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid

    #73205

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    NLS-myc-dL5-2XG4S-mCer3 (MYC Synthetic, Human)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNAz-mBACEflag (C474A/C478A/C482A/C485A)

    Plasmid

    #26736

    Depositor

    Gopal Thinakaran

    Article

    Vetrivel et al J Biol Chem. 2009 Feb 6. 284(6):3793-803

    Insert

    BACE (Bace1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cys 474, 478, 482, and 485 mutated to Ala

    Promoter

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDEST 3xFlag-pcDNA5-FRT/T0-53BP1 28A

    Plasmid

    #52505

    Purpose

    mammalian expression vector of 53BP1 28A mutant

    Depositor

    Daniel Durocher

    Article

    Escribano-Diaz et al Mol Cell. 2013 Mar 7;49(5):872-83. doi:

    Insert

    53BP1 28A (TP53BP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    All 28 SQ/TQ motifs in N-term of 53BP1 have been …

    Promoter

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pcDNA3-LplA(W37V) Coumarin PRIME ligase

    Plasmid

    #25845

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37V
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37V

    Promoter

    Available Since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5'-3xFLAG Kdm1a-exon minimal CMV pCDNA5

    Plasmid

    #212054

    Purpose

    LR vector for integration of Kdm1a-exon into N2a FRT rtTA3 expression cells

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    Kdm1a-exon (Kdm1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 Plk4(Sak) D154A (Nigg HR26)

    Plasmid

    #41164

    Depositor

    Erich Nigg

    Article

    Habedanck et al Nat Cell Biol. 2005 Nov;7(11):1140-6.

    Insert

    Plk4 D154A (PLK4 Human)
    

    Use

    Tags

    3xMyc and PreScission Site

    Expression

    Mammalian

    Mutation

    D154A

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. p5954 pcDNA-Xpress-His-USP15 C269A

    Plasmid

    #23217

    Depositor

    Peter Howley

    Article

    Vos et al J Virol. 2009 Sep . 83(17):8885-92.

    Insert

    USP15 (USP15 Human)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    C269A

    Promoter

    Available Since

    April 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

    Plasmid

    #168192

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-LaG6-(EAAAK)4-OGT(4)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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