We narrowed to 15,730 results for: LEA

1. pCon-IIAl_Con-IIAr

   Plasmid

   #192215

   Purpose

   Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   Consensus II-A Leader and Consensus II-A Locus DNA
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX1-IFl_Con-IFr

   Plasmid

   #192216

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X1)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX2-IFl_Con-IFr

   Plasmid

   #192217

   Purpose

   Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

   Insert

   I-F leader and I-F repeat construct not studied further in this paper (X2)
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEMS2156

   Plasmid

   #158099

   Purpose

   High Copy number plasmid containing SYN1 insert

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   SYN1 (SYN1 Human)
   

   Promoter

   SYN1

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. 6_T7-Lyn-mScarlet3

   Plasmid

   #225933

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   Lyn-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 7_T7-Src64B-mScarlet3

   Plasmid

   #225934

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   Src64B-mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 9_T7-mScarlet3-LactC2

   Plasmid

   #225936

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3-LactC2
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-NRCH-CGBE1

   Plasmid

   #198557

   Purpose

   Expresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH-CGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-NG-ABE8.17-m+V106W

   Plasmid

   #198534

   Purpose

   Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-ABE8.17-m+V106W
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRoC30_Th3.1

    Plasmid

    #188065

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 4 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJRoC30_Th3.7

    Plasmid

    #188066

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJRoC30_Th3.10

    Plasmid

    #188067

    Purpose

    FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

    Insert

    FuncLib-designed high-redox potential laccase from Trametes hirsuta
    

    Tags

    S. cerevisiae evolved α-factor prepro-leader sign…

    Expression

    Yeast

    Mutation

    20 PROSS + 3 FuncLib mutations, described in publ…

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKM-U6-reRNA-mmPCSK9

    Plasmid

    #205634

    Purpose

    reRNA guide targeting Mus Musculus PCSK9

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-mmPCSK9 (Pcsk9 Mouse)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPSt_35Spr-GFP-t35S

    Plasmid

    #203592

    Purpose

    Fully assembled Plant STARR-seq plasmid with the 35S terminator.

    Depositor

    Stan Fields

    Article

    Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

    Inserts

    CaMV 35S promoter + Zm00001d041672 5'UTR
    eGFP
    CaMV 35S terminator
    

    Expression

    Plant

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNAintron-CHIKV-Structural polyprotein with Nluc tagged E2

    Plasmid

    #215699

    Purpose

    Produces the Chikungunya virus structural polyprotein Capsid-E3-NLucE2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV structural polyprotein Capsid-E3-NLucE2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-EF1a-ArgiNLS-miRFP670

    Plasmid

    #220600

    Purpose

    Constitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    miRFP670
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)

    Plasmid

    #220597

    Purpose

    Constitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mVenus-Q69M (ME)
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-ArgiNLS-mKate2

    Plasmid

    #220599

    Purpose

    Constitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mKate2
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-FLEX-ArgiNLS-EGFP

    Plasmid

    #220601

    Purpose

    Cre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Tags

    ArgiNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOTTC1706 - pAAV mGrid1 390F gRNA EF1a EGFP-KASH

    Plasmid

    #131683

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Promoter

    EF1a and mU6

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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