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pLenti6/V5-DEST-NCAPH

Plasmid

#31214

Depositor

Lynda Chin

Article

Scott et al Cancer Cell. 2011 Jul 12;20(1):92-103.

Insert

NCAPH (NCAPH Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDriver

Plasmid

#165149

Purpose

Driver plasmid for PROBER encoding SV40 Large T-antigen

Depositor

Paul Khavari

Article

Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

Insert

SV40 Large T-antigen

Expression

Mammalian

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBAV1K-T5-gfp

Plasmid

#26702

Depositor

Ichiro Matsumura

Article

Bryksin et al PLoS One. 2010 . 5(10):e13244.

Insert

T5lacOlacOeGFP biobrick

Expression

Bacterial

Available Since

Nov. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pSFFV_mNG3A(1-10)

Plasmid

#157992

Purpose

Lentiviral vector of constitutive expression of mNeonGreen3A(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

pSFFV_mNG3K(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

W148L, M150V, K153M, N194K, F204L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEMG-GRIS

Plasmid

#225134

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Gm.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAJM.773

Plasmid

#108527

Purpose

VanRAM + PVanCC-YFP Reporter. Van sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

VanRAM + PVanCC-YFP

Expression

Bacterial

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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Pet28a_Human_H3.3

Plasmid

#42632

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL5-pCMV7.1

Plasmid

#155024

Purpose

N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 5 (CUL5 Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MCP-M-MLV RT∆RNase H

Plasmid

#213749

Purpose

For circular RNA-mediated prime editor using MCP-M-MLV RT∆RNase H in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-RUNX3

Plasmid

#139812

Purpose

doxycycline-inducible expression of Human RUNX3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

RUNX3 (RUNX3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_WT

Plasmid

#81800

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

Plasmid

#51509

Purpose

Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Has Service

AAV1

Insert

tdTomato-T2A-SypEGFP

Use

AAV

Promoter

phSyn1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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cdk1 FRET sensor (2327)

Plasmid

#26064

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cdk1 FRET Sensor

Tags

CFP variant, FLAG, and YFP variant

Expression

Mammalian

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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Oct4-IRES-eGFP-PGK-Neo

Plasmid

#48681

Purpose

For knock-in donor vector by CRISPR/CAS system

Depositor

Rudolf Jaenisch

Article

Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

Insert

Oct4 (Pou5f1 Mouse)

Use

CRISPR

Tags

GFP

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-neo (1795)

Plasmid

#41000

Depositor

Jonathon Pines

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Tags

GSGSGS linker and His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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