We narrowed to 31,373 results for: LIS

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scaffold-linker-U6

Plasmid

#89639

Purpose

construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

Depositor

Wensheng Wei

Article

Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC13-hygro

Plasmid

#231988

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pAd-Wnt3a

Plasmid

#12518

Depositor

Tong-Chuan He

Article

Luo et al J Biol Chem. 2004 Dec 31. 279(53):55958-

Insert

Wnt3A (Wnt3a Mouse)

Use

Adenoviral

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only
pLKO-Tet-On-shRab27a

Plasmid

#120930

Purpose

conditionally knockdown Rab27a in mouse cell lines

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

shRab27a (Rab27a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ZFP36

Plasmid

#178438

Purpose

doxycycline-inducible expression of Human ZFP36 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

ZFP36 (ZFP36 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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RANLS

Plasmid

#50843

Purpose

Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

ddRFP A

Tags

triplicated NLS sequence DPKKKRKV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_Kinprola_PKA-mEGFP-NLS3__WPRE-SV40

Plasmid

#233365

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

Kinprola_PKA-mEGFP-NLS3X

Use

AAV

Tags

mEGFP-NLS3X

Promoter

CAG

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_CAG_NES-Kinprola_PKA-mTagBFP2_WPRE-SV40

Plasmid

#233364

Purpose

CAG driven expression of the PKA activity recorder Kinprola_PKA fused to mTagBFP2 for mammalian cell expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA-mTagBFP2

Use

AAV

Tags

NES and mTagBFP2

Promoter

CAG

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Rb-379-792-pGex2TK

Plasmid

#21748

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-792 of wt Rb …

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CRISPRv2-sgNTC49-puro

Plasmid

#231989

Purpose

Knockout non-targeting control

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

sgRNA with Cas9 and hygromycin resistance

Use

CRISPR and Lentiviral

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ10550

Plasmid

#239269

Purpose

Expresses PYL1-MAVS* for recruiting RNA to the outer mitochondrial membrane via CRISPR-TO

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

PYL1-BFP-MAVS(aa510-540) (MAVS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG eGFP NLS

Plasmid

#170830

Purpose

Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

eGFP

Use

CRISPR, Synthetic Biology, and TALEN

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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HBT-CPK32-Flag

Plasmid

#107432

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Article

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

CPK32 (CPK32 Mustard Weed)

Tags

Flag

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-YE1-BE3-FNLS-CMV-mCherry

Plasmid

#154005

Purpose

Express YE1-BE3-FNLS

Depositor

Hui Yang

Article

Zuo et al Nat Methods. 2020 May 18. pii: 10.1038/s

Insert

YE1-BE3-FNLS

Use

CRISPR

Tags

FLAG

Promoter

CMV

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti CMV SNAP MDC1

Plasmid

#227375

Purpose

SNAP–MDC1 expression

Depositor

David Pellman

Article

Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

Insert

MDC1, mediator of DNA damage checkpoint 1 (MDC1 Human)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

Plasmid

#170823

Purpose

PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Cas13d PPP2CA gRNA2

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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mCardinal-ZO1-C-14

Plasmid

#56179

Purpose

Localization: Tight Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

ZO1 (TJP1 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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IR25a-T2A-QF-AD

Plasmid

#162526

Purpose

Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

Depositor

Leslie Vosshall

Article

Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

Insert

Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm

Expression

Insect

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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