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pHR-HOXA13(+7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145266

Purpose

Mammalian Expression of Nuclear Opto HOXA13(+7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXA13-IDR-mCherry-CRY2-NLS

Plasmid

#145265

Purpose

Mammalian Expression of Nuclear Opto HOXA13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD13-Adel-15A-IDR

Plasmid

#145243

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD13-Adel-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-Adel-15A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-44A-IDR

Plasmid

#145237

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-44A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13-15A-IDR

Plasmid

#145236

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13-15A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2-Adel-IDR

Plasmid

#145234

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2-Adel-IDR (HEY2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-BHLHE41-Adel-IDR

Plasmid

#145228

Purpose

Expresses fusion of GAL4 DNA-binding domain and BHLHE41-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

BHLHE41-Adel-IDR (BHLHE41 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9n)-mHdac1-L2

Plasmid

#122307

Purpose

Expresses sgRNA targeting mouse Hdac1 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Hdac1 (Hdac1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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289aa ELL2

Plasmid

#127266

Purpose

For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu. Contains 289…

Promoter

T7

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2 Met133, 138, 186 to Ileu

Plasmid

#127269

Purpose

For in vitro translation of ELL2 with with internal HA tag and Met133, 138, 186 Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu HA tag after M186

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2 Met133, 138, 186 to Ileu

Plasmid

#127271

Purpose

For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 Met133, 138, 186 to Ileu (ELL2 Human)

Use

In vitro translation

Mutation

Met133, 138, 186 to Ileu with HA tag after 8th Met

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged mid ELL2 M133, 138, 186ILeu

Plasmid

#127276

Purpose

Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Expression

Mammalian

Mutation

M133, 138, 186 to Ileu, HA tag at XbaI after Met …

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Adeno BN

Plasmid

#101823

Purpose

Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1

Depositor

Andrea Ventura

Article

Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)

Use

Adenoviral

Tags

FLAG-Cas9

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
RV-cag-Dio-Kif5c-2A-tdt

Plasmid

#87668

Purpose

retroviral vector, conditional expression membrane Tdtomato and Kif5Cdelta560

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Kif5C-P2A-mTdt (Kif5c Rat)

Use

Retroviral

Tags

palmitylation

Mutation

motor domain aa1-560 present, V40A, E125V (see de…

Promoter

CAG

Available Since

April 19, 2017

Availability

Academic Institutions and Nonprofits only

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pSSRB069_pAC8-hsDDB1dB

Plasmid

#218790

Purpose

Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

6xHis-TEV

Expression

Insect

Mutation

Deleted amino acids 396-705 and replaced with GNG…

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pNK3071

Plasmid

#219755

Purpose

MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH

Expression

Plant

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pYX233-b2-DHFR

Plasmid

#163759

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2-DHFR (Dhfr Mouse, Budding Yeast)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-MCS-MED1long-IDR-YFP-NLS

Plasmid

#145276

Purpose

Mammalian Expression of Nuclear MED1 long IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

MED1-IDR long (MED1 Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-CMVtight-PLXNB2 iOE

Plasmid

#176849

Purpose

lentiviral vector for doxycycline-inducible PLXNB2 overexpression

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

hPLXNB2 (PLXNB2 Human)

Use

Lentiviral

Mutation

full length PLXNB2

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_Puro_Telo_v1

Plasmid

#195138

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Expression

Mammalian

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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