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pCMV-myc-ERK2-MEK1_fusion

Plasmid

#39194

Depositor

Melanie Cobb

Article

Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

Insert

ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-puro_G-CEPIA1er

Plasmid

#164590

Purpose

Stable integration and expression of G-CEPIA1er in mammalian cells

Depositor

Joseph Hyser

Article

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

G-CEPIA1er

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Tags

His6 and TEV site

Expression

Bacterial

Promoter

T5

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPA1L

Plasmid

#19484

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA1L (HSPA1L Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP1

Plasmid

#22596

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP1 (USP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-PheRSa-PheRSb

Plasmid

#224364

Purpose

Express PheRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

PheRS (pheS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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CDK1as_T2A_puromycin

Plasmid

#118595

Purpose

One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

Depositor

William Earnshaw

Article

Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

Insert

CDK1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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