We narrowed to 11,589 results for: EMB
-
Plasmid#138347PurposeExpress guide RNA 1 for Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR v2-sgDnmt1_2
Plasmid#138348PurposeExpress guide RNA 2 for mouse Dnmt1DepositorInsertmouse Dnmt1 sgRNA (Dnmt1 Synthetic)
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
px459-Rheb sgRNA
Plasmid#133768PurposeExpresses Cas9 and human Rheb sgRNADepositorInsertRheb sgRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGASΔN C396/7A-HA
Plasmid#130918PurposeExpresses human cGASΔN (aa160-522)-HA with C396/397A point mutation; Puromycin selection markerDepositorInsertcGASΔN C396/7A
TagsHAExpressionMammalianMutationmutated residues 396 and 397 to alaninesPromoterCMVAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EGFP-bSV1_1-1009
Plasmid#129471PurposeMammalian expression of the N-terminus + central domain of bovine SV1DepositorInsertSupervillin (SVIL Bovine)
ExpressionMammalianMutationcontains amino acids 1-1009PromoterCMVAvailable SinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZIP-hCMV-ZsGreen-P2A-Puro-NT4
Plasmid#123328PurposeLentiviral vector for expression of non-targeting control shRNA #4 from a miR30 embedded casette in the 3'UTR of ZsGreen-P2A-Puro insertDepositorInsertnon-targeting control shRNA embedded in miR30 backbone
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
GSO2/EDA23 X079_pECIA2
Plasmid#115111PurposeBait vector GSO2/EDA23 X079_pECIA2 should be used with prey vector GSO2/EDA23 X079_pECIA14.DepositorInsertAT5G44700 (GSO2 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 p37 deltaUBX
Plasmid#113504PurposeBacterial expression of GST-tagged p37 lacking the UBX domainDepositorInsertp37 (Ubxn2b Mouse)
TagsGST-PreScission protease siteExpressionBacterialMutationdelta 251-331 (stop codon behind AA250)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S55D
Plasmid#114032PurposeInducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell linesDepositorAvailable SinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q0P897
Plasmid#103120PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Q5L8F8
Plasmid#103126PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertQ5L8F8(Cas9 coding gene from Bacteroides fragilis (strain ATCC 25285 / NCTC 9343))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6WZS9
Plasmid#103103PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertE6WZS9(Cas9 coding gene from Nitratifractor salsuginis (strain DSM 16511 / JCM 12458 / E9I37-1))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-F4GDP9
Plasmid#103077PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertF4GDP9(Cas9 coding gene from Alicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-A8LN05
Plasmid#103083PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertA8LN05(Cas9 coding gene from Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12))
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-HLx8
Plasmid#108139PurposeSAC1 with extended linkerDepositorInsertmCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-520):[EAAAR]8:SACM1L(521-587)PromoterCMVAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-mCFP
Plasmid#105142PurposeYeast gene targetingDepositorInsertkanMX6-P81nmt1-mCFP
Tags81nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter81nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-mCFP
Plasmid#105139PurposeYeast gene targetingDepositorInsertkanMX6-P41nmt1-mCFP
Tags41nmt1-mCFPExpressionBacterial and YeastMutationA206KPromoter41nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GL2 shRNA
Plasmid#86083PurposeA lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.DepositorInsertcontrol shRNA
PromoterU6Available SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
Tags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
mini-YFP-hDF-C2F-TM
Plasmid#72600Purposemini human dysferlin (from domain F to transmembrane ) tagged with YFPDepositorInsertdysferlin (DYSF Human)
TagsEYFPExpressionMammalianMutationfrom domain F to transmembrane domainPromoterCMVAvailable SinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only