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We narrowed to 16,181 results for: nes

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Showing: 6701 - 6720 of 16181 results

  1. CGBE1-VRQR (pBM1192)

    Plasmid
    #140254
    Purpose
    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eUNG-BE4max(R33A)_VRQR-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140254-plasmid-map-sequence-id-291779

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  4. pcDNA3-nuc-GsCT

    Plasmid
    #205517
    Purpose
    Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).
    Depositor
    Jin Zhang
    Publication
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuc-GsCT (GNAS Human, Synthetic)
    Tags
    Histone H2A and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205517-plasmid-map-sequence-id-409404

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  8. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Publication
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

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  11. nCas9-UGI-NLS-P2A-EGFP (pJUL1001)

    Plasmid
    #123611
    Purpose
    CAG promoter expression plasmid for hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (BE3 control without rAPOBEC1).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9-UGI-NLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A in SpCas9
    Promoter
    CAG
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    123611-plasmid-map-sequence-id-239339

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  14. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid
    #153062
    Purpose
    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)
    Depositor
    Silvia Monticelli
    Publication
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Mutagenesis to insert a stop codon at aa 298 in B…
    Promoter
    EF1α
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153062-plasmid-map-sequence-id-304636

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  17. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  18. pATP416-­pluxO5-­pphlO6-­crtYBI

    Plasmid
    #165977
    Purpose
    Expresses carotenoid biosynthesis gene in response to homoserine lactone and 2,4-diacetylphloroglucinol in yeast expressing PhlTA and LuxTA
    Depositor
    Jun Ishii
    Publication
    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:
    Inserts
    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    C66A, C1359T and C1281A, G1698A
    Promoter
    Synthetic promoter (pluxO5), Synthetic promoter (…
    Available since
    March 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    165977-plasmid-map-sequence-id-328856

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Showing: 6701 - 6720 of 16181 results