We narrowed to 9,345 results for: sacs

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pTH737-CEN-maxHIS3

Plasmid

#38229

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast, Synthetic)

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFP84

Plasmid

#83542

Purpose

Used to evaluate the expression output of SSA1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

SSA1 promoter-yEGFP

Expression

Yeast

Promoter

SSA1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RLuc8C-FHA1-NES

Plasmid

#138210

Purpose

Encodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

RLuc8C-FHA1-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0113

Plasmid

#117552

Purpose

Level 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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A102 ΔAmpH Δ2x+ve

Plasmid

#129484

Purpose

Expression of Glo3 ΔAmpH lacking the COPI-binding motif in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pKV005

Plasmid

#64763

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable Myc tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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BAS291 C terminal mKO2 kanR

Plasmid

#74086

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pKP4010

Plasmid

#113336

Purpose

Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Inserts

Artificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase

Tags

3X-FLAG/8X-Gly linker

Expression

Yeast

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pGS1011

Plasmid

#63783

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

Strep-tag II followed by TRP1 selection marker

Use

E. coli cloning vector

Tags

Strep-tag II

Promoter

none

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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BAS290 C terminal E2C natR

Plasmid

#74085

Purpose

S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

E2Crimson

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Yeast

Promoter

native

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-Purg1-GST

Plasmid

#39352

Depositor

Jurg Bahler

Article

Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

Insert

urg1 promoter (urg1, urg1 Fission Yeast)

Use

Yeast genomic targeting

Tags

GST and kanMX6 module - reverse orientation

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pCfB2801

Plasmid

#63656

Purpose

pCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Insert

GFP

Use

Cre/Lox

Expression

Yeast

Promoter

TEF1 promoter

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pFP122

Plasmid

#21669

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Mutation

The second lacZ sequence has been modified, the E…

Available Since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
AB.pCCL.sin.cPPT.U6.miR-24-Decoy.hPGK.GFP.WPRE

Plasmid

#46602

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-24 Decoy (Mir24-1 Human, Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only
pRY1004

Plasmid

#21788

Depositor

Richard Yu

Article

Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

Insert

Dig1-YFP (DIG1 Budding Yeast, A. victoria)

Tags

YFP

Expression

Yeast

Mutation

contains 5' 1000bp UTR (Dig1 promoter) Di…

Available Since

Sept. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pET151-Vta1(280-330) S278C

Plasmid

#87739

Purpose

Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-277, mutated Ser 278 to Cys

Promoter

T7

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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AB.pCCL.sin.cPPT.GFP.miR-370-5p.sensor.PGK.dNGFR.WPRE

Plasmid

#85854

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-370-5p Sensor (MIR370 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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