We narrowed to 9,442 results for: pho

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pMx/ATP8B2(E171Q)-HA

Plasmid

#209254

Purpose

Mammalian expression of ATP8B2 mutant E171Q

Depositor

Hye-Won Shin

Article

Takatsu et al Mol Cell Biol. 2024 Sep 2:1-16. doi: 10.

Insert

ATP8B2 (ATP8B2 Human)

Use

Retroviral

Tags

HA

Mutation

E171Q

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-43 mRuby K84ONBK

Plasmid

#216226

Purpose

TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

Transactive response DNA binding protein of 43 kDa (TARDBP Human)

Expression

Mammalian

Mutation

Lysine 84 mutated to a TAG stop codon

Promoter

CMV Promoter

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-Exon3

Plasmid

#209075

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-Exon3 HDRT (PRF1 Human)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCF702

Plasmid

#209650

Purpose

Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

Depositor

Bastian Molitor

Article

Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

Inserts

Downstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMX-IG-ATG3 (H262A)

Plasmid

#212027

Purpose

Expresses untagged ATG3 (H262A) in mammalian cells

Depositor

Michael Lazarou

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET23a-H6-TEV-AncSZ

Plasmid

#214235

Purpose

Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

Depositor

Neel Shah

Article

Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

Insert

Syk kinase (SYK Human)

Tags

6xHis

Expression

Bacterial

Mutation

Sequence gained through ancestral reconstruction …

Promoter

T7

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-ST-P2A-pSC-CAAX

Plasmid

#207637

Purpose

A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX

Expression

Mammalian

Mutation

Amber stop codon at SC's critical lysine

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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Shim_ProRS_TP_GFP_pK7FWG2

Plasmid

#202651

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic ProRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_ThrRS_TP_GFP_pK7FWG2

Plasmid

#202652

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic ThrRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_TrpRS_TP_GFP_pK7FWG2

Plasmid

#202653

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic TrpRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_AspRS_TP_GFP_pK7FWG2

Plasmid

#202647

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic AspRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_GlyRS_TP_GFP_pK7FWG2

Plasmid

#202648

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic GlyRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_AsnRS_TP_GFP_pK7FWG2

Plasmid

#202649

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic AsnRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Shim_MetRS_TP_GFP_pK7FWG2

Plasmid

#202650

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative cytosolic MetRS

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pWalium Htt19Q-mCherry

Plasmid

#201248

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Branco-Santos et al Hum Mol Genet. 2017 Oct 1;26(19):3763-37

Insert

Huntingtin (HTT Human)

Expression

Insect

Available Since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA1

Plasmid

#201622

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-SNRPA_sgRNA2

Plasmid

#201623

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SNRPA (SNRPA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199D]-FLAG

Plasmid

#199315

Purpose

mutated phosphorylation site (kinase acitivity permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Aspartic Acid

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S199A]-FLAG

Plasmid

#199313

Purpose

mutated phosphorylation site (kinase acitivity inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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