We narrowed to 9,680 results for: pho

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pMXs-puro HA-Atg14*

Plasmid

#38265

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

Plasmid

#99221

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos3.1

Expression

Bacterial

Mutation

A69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMY-eGFP-P2A-PuroR-HA

Plasmid

#163343

Purpose

Retroviral vector to express cytosolic GFP and an HA-tagged puromycin selection marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Insert

GFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMY-MCS-P2A-Ly6G

Plasmid

#163353

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Ly6G surface marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-miniSOG-VAMP2-citrine

Plasmid

#50969

Purpose

AAV2 transfer vector containing the InSynC (miniSOG-VAMP2-citrine) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Insert

miniSOG-VAMP2-citrine (Vamp2 Arabdopsis thaliana, Mouse)

Use

AAV

Tags

citrine and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCZGY 1703 tomm-20N::miniSOG(426Cys) in pENTR[1-2]

Plasmid

#66781

Purpose

Gly426 of miniSOG was mutated to 426Cys in tomm-20N::miniSOG

Depositor

Yishi Jin

Article

Qi et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

miniSOG

Tags

tomm-20 (N terminal 55 aa)

Expression

Worm

Mutation

G426C

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

Plasmid

#188992

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCerulean3-LaminB1-10

Plasmid

#55425

Purpose

Localization: Nuclear Envelope, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

LaminB1 (LMNB1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S227D]-FLAG

Plasmid

#199320

Purpose

mutated phosphorylation site (MLKL interaction permanent active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Aspartic Acid

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIPK3[S227A]-FLAG

Plasmid

#199318

Purpose

mutated phosphorylation site (MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 227 to Alanine

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-UVRAG

Plasmid

#38267

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV radiation resistance-associated gene protein (UVRAG Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
mCerulean3-Fibrillarin-7

Plasmid

#55419

Purpose

Localization: Nucleoli, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Fibrillarin (FBL Human)

Tags

mCerulean3

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Nov. 3, 2014

Availability

Academic Institutions and Nonprofits only

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mChF-Cav1s

Plasmid

#171464

Purpose

Expresses mChF-Cav1s, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

mCherry-FKBP12-Caveolin1(61-178 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(GFP)-PGKpuro2ABFP-W

Plasmid

#200475

Purpose

Lentiviral vector expressing gRNA targeting GFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

GFP

Use

Lentiviral

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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MEN2A-V5

Plasmid

#139629

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2B-V5

Plasmid

#139630

Purpose

V5-His-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2B (RET Human)

Tags

3xV5-6xHis

Expression

Mammalian

Mutation

M918T

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Axin1_HA in pRK5

Plasmid

#154220

Purpose

C-terminal HA-tagged AXIN1 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

AXIN1 (AXIN1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-nsp1-c3xFLAG

Plasmid

#215694

Purpose

Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp1

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp2-c3xFLAG

Plasmid

#215695

Purpose

Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp2

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNAintron-CHIKV-nsp4-c3xFLAG

Plasmid

#215697

Purpose

Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV nsp4

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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