19,660 results

1. PyronicSF/pcDNA3.1(-)

   Plasmid

   #124812

   Purpose

   Highly resposive GFP-based pyruvate nanosensor.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Insert

   PyronicSF
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CaV2.1 (pcDNA6)

   Plasmid

   #26578

   Depositor

   Diane Lipscombe

   Article

   Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

   Insert

   Cacna1a (Cacna1a Mouse)
   

   Tags

   His6 and V5

   Expression

   Mammalian

   Available Since

   Nov. 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiCRISPR - EGFP sgRNA 2

   Plasmid

   #51761

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

   Inserts

   Cas9
   Puromycin resistance
   EGFP sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EFS and U6

   Available Since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG5 PPAR alpha

   Plasmid

   #22751

   Depositor

   Bruce Spiegelman

   Insert

   PPARalpha (Ppara Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pECE

   Plasmid

   #26453

   Depositor

   William Rutter

   Article

   Ellis et al Cell. 1986 Jun 6;45(5):721-32.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE hBRM

   Plasmid

   #1961

   Depositor

   Robert Kingston

   Article

   Sif et al Genes Dev 2001 Mar 1;15(5):603-18.

   Insert

   BRM (SMARCA2 Human)
   

   Use

   Retroviral

   Tags

   flag

   Expression

   Mammalian

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKM468-EGFP

   Plasmid

   #108434

   Purpose

   ORBIT integrating plasmid for C-terminal tagging with EGFP.

   Depositor

   Kenan Murphy

   Article

   Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

   Insert

   attB-eGFP-4xGly-TEV-Flag-6xHis
   

   Use

   Orbit integrating plasmid

   Available Since

   May 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT3-Neo-EF1a-GFP

   Plasmid

   #69134

   Purpose

   Sleeping Beaquty (SB) vector encoding G418 resistance and GFP from two separate promoters

   Depositor

   Martin Bonamino

   Article

   Chicaybam et al Front Bioeng Biotechnol. 2017 Jan 23;4:9

   Type

   Empty backbone

   Use

   Sleeping beauty transposon

   Expression

   Mammalian

   Promoter

   U3MSCV

   Available Since

   Jan. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST17-UbE2G1

    Plasmid

    #15790

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

11. TIGRE docking site targeting vector

    Plasmid

    #61580

    Purpose

    Create a docking site in the mouse TIGRE genomic locus that allows modification in ES cells by Flp recombinase-mediated cassette exchange

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Type

    Empty backbone

    Use

    Mouse Targeting

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR

    Plasmid

    #104567

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activator

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    human codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
    

    Use

    CRISPR

    Tags

    NLS-3xHA-VPR

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-JunWT-Myc

    Plasmid

    #47443

    Purpose

    mammalian expression of WT c-Jun

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun (Jun Mouse)
    

    Tags

    Flag and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

    Plasmid

    #134860

    Purpose

    Expresses EGFP-tagged recycling endosome marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAB11B (RAB11B Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin1 (CUL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO-p53-shRNA-941

    Plasmid

    #25637

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-941 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCVpuro-mTagBFP2

    Plasmid

    #96935

    Purpose

    Retroviral plasmid containing mTagBFP2 for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    Monomeric Tag Blue Fluorescent Protein
    

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPCX mito Grx1-roGFP2

    Plasmid

    #64977

    Purpose

    Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCBC-MT2T3

    Plasmid

    #50594

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Insert

    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    

    Use

    CRISPR; Pcr template

    Expression

    Plant

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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