20,066 results
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pRGE32
Plasmid#63159PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplastDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV RasV12 Neo (w108-1)
Plasmid#22259DepositorAvailable SinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-R1441C
Plasmid#25363DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationArginine 1441 mutated to CysteineAvailable SinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcdna3.1 hciap1
Plasmid#8311DepositorAvailable SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-V5-PTK2B
Plasmid#127233PurposeEukaryotic expression of PTK2BDepositorInsertPTK2B
TagsV5ExpressionMammalianPromoterCMVAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG human DEPTOR
Plasmid#21334DepositorInsertDEPTOR (DEPTOR Human)
TagsFLAGExpressionMammalianMutationN204S and S389N mutations (numbering according to…Available SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAd/WT1-IRES-nAmCyan
Plasmid#29756DepositorInsertWilms tumor 1 (WT1 Human)
TagsAmCyan, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; AmCyan is nuclear localized; WT1 an…Available SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9_KRAB_2A_EGFP
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-HA-NEK7
Plasmid#75142PurposeExpresses N-terminal HA-tagged NEK7 in mammalian cellsDepositorInsertNIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
TagsHAExpressionMammalianPromoterCMVAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyLigase
Plasmid#51722PurposePlasmid for recombinant expression in bacteria of SpyLigase. The Howarth lab has created two improved peptide ligation enzymes. See depositor comments below!DepositorInsertSpyLigase
TagsHis6 TagExpressionBacterialPromoterT7Available SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
HR_G3BP1-V5-APEX2-GFP
Plasmid#105284Purposehomologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
UseHuman homologous recombination donorAvailable SinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsXRN1
Plasmid#66596Purposeexpresses lambdaN-HA-tagged XRN1 in mammalian cellsDepositorAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-HSP70
Plasmid#163790PurposeExpresses mScarlet-Hsp70 in mammalian cellsDepositorAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKT-PH-GFP
Plasmid#18836DepositorInsertAKT-PH domain (AKT1 Human)
TagsEGFPExpressionMammalianMutationcontains amino acids 1-148 (compared to GenBank r…PromoterCMVAvailable SinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-beta-HA-BRCA2
Plasmid#133553PurposeMammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORFDepositorInsertBRCA2 (BRCA2 Human)
ExpressionMammalianAvailable SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pyEvolvR (enCas9-PolI5M)
Plasmid#158073PurposeExpresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI5M
ExpressionBacterial and YeastAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
3120 pSFFV-neo Bcl-XL
Plasmid#8749DepositorInsertBcl-xl (BCL2L1 Human)
ExpressionMammalianAvailable SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RLuc
Plasmid#83282Purposerecombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)DepositorInsertRenilla Luciferase
UseAAVExpressionMammalianPromoterCAGAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only
