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pFX-Lifeact-Sirius

Plasmid

#174475

Purpose

Express Sirius with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-QZ213

Plasmid

#215484

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 14, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-AD-CYH2

Plasmid

#215483

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-AD-AR68

Plasmid

#215485

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-DB

Plasmid

#215482

Purpose

Yeast expression plasmid

Depositor

David E Hill

Article

Luck et al Nature. 2020 Apr;580(7803):402-408. doi:

Type

Empty backbone

Expression

Yeast

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pHflu1

Plasmid

#203878

Purpose

pSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transfer

Depositor

Bogumil Karas

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-PHB-4

Plasmid

#122231

Purpose

Expresses shRNA targeting the 3' UTR of human PHB

Depositor

Alex Kentsis

Article

Ortiz et al JCI Insight. 2019 Aug 8;4(15). pii: 1270

Insert

PHB (PHB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI3hyp

Plasmid

#246636

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3hyp under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3hyp

Expression

Insect

Promoter

hsp70

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI3hyp

Plasmid

#246640

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3hyp expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3hyp

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pJFRC7-JEDI3sub

Plasmid

#246623

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub under the promoter hsp70 for Drosophila (insect) expression

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Expression

Insect

Promoter

hsp70

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI3sub

Plasmid

#246627

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI3hyp-WPRE

Plasmid

#246632

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3hyp in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3hyp

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-JEDI3hyp

Plasmid

#246634

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3hyp under the HuC(elavl3) promoter for pan neuronal expression in zebrafish

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3hyp

Use

Zebrafish expression

Promoter

HuC(elavl3)

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI3sub-WPRE

Plasmid

#246619

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub in an AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-JEDI3sub

Plasmid

#246621

Purpose

Genetically encoded voltage indicator (GEVI) JEDI3sub under the HuC(elavl3) promoter for pan neuronal expression in zebrafish

Depositor

Francois St-Pierre

Article

Land et al Nat Methods. 2026 Apr 15. doi: 10.1038/s

Insert

JEDI3sub

Use

Zebrafish expression

Promoter

HuC(elavl3)

Available Since

April 23, 2026

Availability

Academic Institutions and Nonprofits only

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px458-AMBRA1 (optimized for N-terminal knock-in)

Plasmid

#172608

Purpose

Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

gRNA against AMBRA1 (AMBRA1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1602

Plasmid

#29305

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748) and de Leeuw et al., MTM, 2014 (PMID: 24761428).

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple131 (MKI67 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1600

Plasmid

#29274

Purpose

Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).Pleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PN

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple129 (MKI67 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

Intron-LacZ-NLS

Available Since

Nov. 4, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1127

Plasmid

#29049

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple58 (DDC Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1225

Plasmid

#29113

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Article

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple243 (VIM Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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