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pPOTv7-blast-mCardinal

Plasmid

#179796

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCardinal

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pICSL50009

Plasmid

#50309

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HA tag (6x Human influenza hemagglutinin)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mNG

Plasmid

#179817

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10FLAG

Plasmid

#181940

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xFLAG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDiGi

Plasmid

#59323

Purpose

Inducible expression of dsRed and GFP in bacteria

Depositor

Sophie Helaine, David Holden

Article

Helaine et al Proc Natl Acad Sci U S A. 2010 Feb 23;10

Inserts

EGFP
dsRed

Expression

Bacterial

Promoter

Pbad and Plac

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-Kan

Plasmid

#44906

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Expression

Yeast

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-CLIPtag

Plasmid

#179787

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

CLIP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mNG

Plasmid

#179819

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSFFV_CloGFP(1-10)

Plasmid

#157994

Purpose

Lentiviral vector of constitutive expression of CloGFP(1-10)

Depositor

Bo Huang

Article

Zhou et al PLoS One. 2020 Nov 23;15(11):e0242592. d

Insert

CloGFP(1-10)

Use

Lentiviral

Expression

Mammalian

Mutation

G32Q, F46L, K101R, S202L

Promoter

SFFV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBT317_pET-PhiC31o-pA

Plasmid

#52491

Purpose

for preparation of PhiC31o mRNA

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

PhiC31o integrase

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL.SP146.gp47.eGFP

Plasmid

#30475

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp47

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

SNAP

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Gm

Plasmid

#105232

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pME-BrainbowTEC

Plasmid

#82405

Purpose

Gateway-compatible Brainbow-1.0 with fluorophores tdTomato-myc/EGFP/E2Crimson-HA

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Inserts

tdTomatoMYC fusion
EGFP
E2CrimsonHA

Tags

HA and MYC

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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FR_luc

Plasmid

#31098

Depositor

Gerhart Ryffel

Article

Thomas et al Nucleic Acids Res. 2004 . 32(19):e150.

Insert

firefly luciferase

Use

Flp/frt

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
pPOTv7-blast-HaloTag

Plasmid

#181953

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

HaloTag

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10HA

Plasmid

#181941

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xHA

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Kan

Plasmid

#105233

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL.SP146.gp91.eGFP

Plasmid

#30474

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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