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We narrowed to 9,071 results for: sgRNA

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Showing: 6741 - 6760 of 9071 results

  1. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid
    #225755
    Purpose
    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection
    Depositor
    Paul Blainey
    Publication
    Walton et al bioRxiv [Preprint]. 2024 Mar 17:2024.03.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    225755-plasmid-map-sequence-id-446065

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  2. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  3. pBZ202-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Sa163RT-NLS-T2A-mCherry

    Plasmid
    #170184
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Sa163 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Sa163 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Publication
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Sa163 RT
    Retron Sa163 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170184-plasmid-map-sequence-id-342530

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  5. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  6. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    92114-plasmid-map-sequence-id-197615

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  7. pU6:2-U6:3-tevopreq1

    Plasmid
    #190910
    Purpose
    Expression of nicking sgRNA and epegRNA under control of separate Drosophila U6:2 and U6:3 promoters. Can be used to generate transgenic flies with vermillion+ selection.
    Depositor
    Norbert Perrimon
    Insert
    Empty Backbone
    Use
    CRISPR
    Expression
    Insect
    Available since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190910-plasmid-map-sequence-id-373034

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  8. pICSL4723_ Pi21

    Plasmid
    #126892
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126892-plasmid-map-sequence-id-249677

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  9. pICSL4723_ CUL3

    Plasmid
    #126891
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    126891-plasmid-map-sequence-id-250181

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  10. pICSL4723_ PLDbeta1

    Plasmid
    #126890
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126890-plasmid-map-sequence-id-250163

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  11. pICSL4723_ WRKY28_1

    Plasmid
    #126887
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126887-plasmid-map-sequence-id-249685

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  12. pICSL4723_ HDT701

    Plasmid
    #126886
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126886-plasmid-map-sequence-id-249696

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  13. pICSL4723_ Rac5

    Plasmid
    #126885
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126885-plasmid-map-sequence-id-249710

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  14. pICSL4723_ Rac4

    Plasmid
    #126884
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126884-plasmid-map-sequence-id-249713

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  15. pENTRY4_ Dja6_2

    Plasmid
    #126895
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126895-plasmid-map-sequence-id-250173

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  16. pENTRY4_ Rac4

    Plasmid
    #126896
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126896-plasmid-map-sequence-id-250171

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  17. pENTRY4_ CUL3

    Plasmid
    #126903
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126903-plasmid-map-sequence-id-250107

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  18. pENTRY4_Rac5

    Plasmid
    #126897
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126897-plasmid-map-sequence-id-250096

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  19. pENTRY4_ WRKY28_1

    Plasmid
    #126899
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126899-plasmid-map-sequence-id-250041

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  20. pENTRY4_ WRKY28_2

    Plasmid
    #126900
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126900-plasmid-map-sequence-id-249965

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Showing: 6741 - 6760 of 9071 results