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pGEX Lnk-SH2

Plasmid

#46451

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lnk (Sh2b3 Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 325-466

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Slap2-SH2

Plasmid

#46501

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap2 (SLA2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 84-197

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry

Plasmid

#75133

Purpose

Retroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

shBrd9_1116

Use

Retroviral

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only
mOrange2-Vimentin-7

Plasmid

#57977

Purpose

Localization: Intermediate Filaments, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Vimentin (VIM Human)

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only
mRuby2-Tubulin-6

Plasmid

#55914

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Tubulin (TUBA1B Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
FusionRed-Sit-N-15

Plasmid

#56133

Purpose

Localization: Golgi TGN, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Sit (ST6GAL1 Human)

Tags

FusionRed

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pGEX p85b(NC)-SH2

Plasmid

#46468

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85b(NC) (PIK3R2 Bovine)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 311-717

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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-111 hTF m3

Plasmid

#15450

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 EGR-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Nck2-SH2

Plasmid

#46458

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

NM_001004720.2 (NCK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 274-380

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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33-G-X1-pEF1-puro

Plasmid

#111604

Purpose

Express human 33-G-X1 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 and GARP chimeria (NRROS Human)

Tags

Flag tag

Expression

Mammalian

Promoter

EF1a

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Tem6-SH2

Plasmid

#46523

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tem6 (TNS3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1171-1291

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-Scara3-IRES-BFP

Plasmid

#117265

Purpose

Retroviral overexpression of Scara3

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Scara3 (Scara3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE

Plasmid

#168108

Purpose

Tol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoR

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Type

Empty backbone

Use

CRISPR and Cre/Lox

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Talin-IBS2-7

Plasmid

#57424

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Talin IBS2 (Tln1 Mouse)

Tags

mEos2

Expression

Mammalian

Mutation

Talin Integrin Binding Site

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Vrap-SH2

Plasmid

#46531

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vrap (SH2D2A Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 93-192

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-RCK-Y1699C

Plasmid

#25067

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Contains only the ROC-COR-Kinase domains aa 1329…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pGEX BRDG1-SH2

Plasmid

#46410

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

BRDG1 (STAP1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 167-283

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
-111 hTF m4

Plasmid

#15451

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 and EGR-1 sites mutated

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pGEX Shb-SH2

Plasmid

#46485

Depositor

Bruce Mayer, Michael Welsh

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Shb (SHB Human)

Tags

GST, HA, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 401-508

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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