We narrowed to 9,742 results for: Tec

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pGS-M1-BRG1-3xFLAG

Plasmid

#109188

Purpose

Plasmid for expression of C-terminally 3xFLAG-tagged BRG1 under the control of a p10 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Jayaprakash et al Mol Cell Biochem. 2019 Nov;461(1-2):171-

Insert

BRG1 (SMARCA4 Human)

Tags

3xFLAG

Expression

Insect

Promoter

p10

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Th3.14

Plasmid

#188068

Purpose

FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

FuncLib-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 PROSS + 4 FuncLib mutations, described in publ…

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-FLAG-human cGAS-HA

Plasmid

#186849

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

FLAG and HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec22b-shR

Plasmid

#208358

Purpose

Mammalian expression of fluorescent N-terminally tagged shRNA resistant Sec22b

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

Sec22b (Sec22b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

four silent mutations introduced to render shRNA …

Promoter

CMV

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKSR1-CA3-GS-EGFP (C-KSR-GS)

Plasmid

#217757

Purpose

Fluorescent reporter for ceramide (mammalian expression)

Depositor

Paula Nunes-Hasler

Article

Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

Insert

Kinase suppressor of ras 1 CA3 domain (KSR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CA3 domain aa 317-400 with GGSSGGGGA linker

Promoter

CMV

Available Since

June 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-PARP1-delM43;F44I

Plasmid

#211579

Purpose

CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

Depositor

Chris Lord

Article

Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

Insert

PARP1 (PARP1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Contains deletion of M43 and F44I

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

Plasmid

#191397

Purpose

sgRNA targeting alpha-satellites on human chromosome 9

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

sgRNA targeting alpha satellites on human chromosome 9

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-p65

Plasmid

#183929

Purpose

Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-p65 (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP ratAPOBEC1 L173A/G227A

Plasmid

#167169

Purpose

Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP-INPP5E(D556A)

Plasmid

#183678

Purpose

Recruitable human type IV 5-phosphatase enzyme (catalytically inactive)

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

INPP5E (INPP5E Human)

Tags

mRFP-FKBP12(1-108)

Expression

Mammalian

Mutation

C641A, D556A

Promoter

CMV

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113D

Plasmid

#107161

Purpose

For mammalian expression of Flag-hPKL S113D

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113D (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to aspartate

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-AICD-NES-IRES-hrGFP

Plasmid

#107544

Purpose

AAV-mediated expression of AICD (last 50 amino acids of APP) with Nuclear Export Signal (NES: CTCCCTCCACTAGAGCGACTAACCTTA) at C-term end and IRES-mediated co-expression of hrGFP

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

AICD-NES (APP Human)

Use

AAV

Mutation

None

Promoter

CMV

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS2_HindIII

Plasmid

#65666

Purpose

FLAG-HA expression vector with RBPMS2 carrying mutation to introduce HindIII site

Depositor

Thomas Tuschl

Article

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS2 (RBPMS2 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced HindIII site (final goal to exchange F…

Promoter

CMV, 2x Tet

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25-VWA

Plasmid

#64772

Purpose

Mammalian expression of Flag-tagged Med25-VWA

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VWA (MED25 Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS302D

Plasmid

#62529

Purpose

Serine 302 of Drosha is mutated to aspartic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 302 changed to aspartic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 302 to aspartic acid

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
GFP-DroshaS300E

Plasmid

#62528

Purpose

Serine 300 of Drosha is mutated to glutamic acid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with serine 300 changed to glutamic acid (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

changed serine 300 to glutamic acid

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only