We narrowed to 1,090 results for: rbis

1. pT3TS-RfxCas13d-NLS-HA

   Plasmid

   #141321

   Purpose

   Plasmid to carry out IVT of RfxCas13d-NLS (human codon-optimized)

   Depositor

   Ariel Bazzini, Miguel Angel Moreno-Mateos

   Article

   Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

   Insert

   Rfx-Cas13d (NLS-RfxCas13d-NLS)
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-T7-TadCBEd-SpCas9-P2A-EGFP (BKS327)

   Plasmid

   #223123

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpCas9 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpCas9-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpCas9=D10A

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-PEmax-P2A-EGFP (LM1589)

   Plasmid

   #223136

   Purpose

   pCMV and pT7 Human expression plasmid for PEmax(nSpCas9(H840A)-M-MMLV_RT**)-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized PEmax-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS and NLS(SV40)-NLS(cMyc)-P2A-EGFP

   Expression

   Mammalian

   Mutation

   M-MLV mutations from PE2 (Anzalone et al. Nature …

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT/TO-3*Flag-APEX2 N-term FBL

   Plasmid

   #187577

   Purpose

   Express FLAG epitope and APEX2-tagged FBL fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   FBL (FBL Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-3*Flag-APEX2 C-term EWSR1

   Plasmid

   #187586

   Purpose

   Express FLAG epitope and APEX2-tagged EWSR1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. iGFP-beta-actin

   Plasmid

   #231553

   Purpose

   Mammalian expression of human beta actin fused intramolecularly to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP (intramolecular)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-N1_c-SRC

   Plasmid

   #171850

   Purpose

   pEGFP-N1_c-SRC

   Depositor

   Michele Pagano

   Article

   Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

   Insert

   c-SRC (SRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Added a linker sequence in 3' of c-SRC: GSGS

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-2xFLAG-2xSTREP-CCND1-IRES-mCherry

   Plasmid

   #172640

   Purpose

   Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D1 and mCherry in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Lentiviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-49: MYL2-mEGFP

   Plasmid

   #114414

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. iGFP-gamma-actin

    Plasmid

    #231554

    Purpose

    Mammalian expression of human gamma actin fused intramolecularly to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTG1 (ACTG1 Human)
    

    Use

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX304_zeo_mmFbxw7a

    Plasmid

    #160102

    Purpose

    Expresses murine Fbxw7 alpha in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

    Plasmid

    #172611

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDKN1B (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR 223 VPS4A

    Plasmid

    #169319

    Purpose

    Gateway entry vector for human VPS4A ORF

    Depositor

    Francisca Vazquez

    Article

    Neggers et al Cell Rep. 2020 Dec 15;33(11):108493. doi

    Insert

    VPS4A (VPS4A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-TDP-43ΔNLS-mClover3

    Plasmid

    #214916

    Purpose

    inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    TDP-43ΔNLS (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNTK human MBD3 PGK-Neo-pA flox

    Plasmid

    #52362

    Purpose

    Targeting vector for Human MBD3 conditional knockout. Also creates hypomorphic alleles.

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    MBD3 (MBD3 Human)
    

    Use

    Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMSCVpuro-Mito-DsRed1

    Plasmid

    #87379

    Purpose

    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    TXN2 mitochondrial transit peptide (TXN2 Human)
    

    Use

    Retroviral

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    First 60 aa only

    Promoter

    Retroviral 5' LTR promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52379

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    OCT4

    Available since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AICSDP-46: MYL7-mEGFP

    Plasmid

    #114413

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL7, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL7 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL7 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe_puro_DEST_Flag_MDS1

    Plasmid

    #45258

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    MDS1 (MECOM, MDS1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pLVX-2xFLAG-2xSTREP-CCND3-IRES-mCherry

    Plasmid

    #172620

    Purpose

    Lentiviral bicistronic vector for the constitutive co-expression of 2xFLAG-2xSTREP-tagged cyclin D3 and mCherry in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Lentiviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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