2,226 results

1. pGP-CMV-GCaMP6-210

   Plasmid

   #91778

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   Douglas Kim, GENIE Project

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-210
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA TrkC

   Plasmid

   #24089

   Depositor

   Moses Chao

   Article

   Yano et al J Neurosci. 2001 Feb 1. 21(3):RC125.

   Insert

   TrkC (Ntrk3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-C1-rSyntaxin-3a

   Plasmid

   #195206

   Purpose

   Mammalian expression vector for GFP tagged Syntaxin-3a.

   Depositor

   Thomas Weimbs, David Bryant

   Article

   Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

   Insert

   STX3A (Stx3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSW GAP43-jRGECO1a P2A Syp-Halo

   Plasmid

   #185565

   Purpose

   Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

   Depositor

   Edwin Chapman

   Article

   Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

   Insert

   GAP43-jRGECO1a P2A Syp-Halo
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. mMaple-OMP25

   Plasmid

   #141151

   Purpose

   Photoconvertible fluorescent protein targeted to the outer mitochondrial membrane (OMM) in mammalian cells.

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat)
   

   Use

   Tags

   mMaple

   Expression

   Mammalian

   Mutation

   OMP25 c terminus from addgene plasmid #69598

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE GFP-Bsn KI

   Plasmid

   #139664

   Purpose

   Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFSynW Syt1 I367T IRES mRuby3

   Plasmid

   #170642

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   I367T

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-CIB1-mRFP1-MP

   Plasmid

   #58367

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV312.3

   Plasmid

   #119944

   Purpose

   Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

   Depositor

   Gerald Schwank

   Article

   Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

   Insert

   C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-ALK7 K222R

    Plasmid

    #80887

    Purpose

    mammalian expression of ALK7 K222R

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K222R

    Promoter

    CMV

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-Cx43-7

    Plasmid

    #55023

    Purpose

    Localization: Gap Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-MyosinVb-Ctail

    Plasmid

    #110170

    Purpose

    Dominant negative effect by competing with MyosinVb C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Vb (Myo5b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    amino acids 1262-1670

    Promoter

    CMV

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1-CAMKIIbeta(T287D)

    Plasmid

    #21223

    Depositor

    Tobias Meyer

    Article

    Shen et al Science. 1999 Apr 2. 284(5411):162-6.

    Insert

    CAMKIIbeta (Camk2b Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    CAMKIIbeta(T287D) Lab plasmid CF 0001.

    Promoter

    Available since

    July 29, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pMT2 Beta1b GFP

    Plasmid

    #89893

    Purpose

    Mammalian expression of Beta1b GFP

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP sequence inserted just before the Stop codon

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-His-3C-irisin

    Plasmid

    #122612

    Purpose

    Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

    Depositor

    Harold Erickson

    Article

    Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

    Insert

    irisin (ectodomain of FNDC5) (Fndc5 Bovine, Human, Rat, Mouse)
    

    Use

    Tags

    Nt: histag-3C protease site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 polymerase

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJL-SaBE3

    Plasmid

    #85169

    Purpose

    Expresses SaBE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    SaBE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMT2 Beta1b mCherry

    Plasmid

    #89892

    Purpose

    Mammalian expression of Beta1b mCherry

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry sequence inserted just before the Stop co…

    Promoter

    adenovirus major late promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCF CREB M1

    Plasmid

    #22969

    Depositor

    Marc Montminy

    Article

    Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

    Insert

    CREB (Creb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 133 to Alanine

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-N2-PKCgamma

    Plasmid

    #21204

    Depositor

    Tobias Meyer

    Article

    Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

    Insert

    PKC gamma (Prkcg Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    PKC gamma (rat). (Lab plasmid WC 0013)

    Promoter

    Available since

    July 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-Neo-HKIImut

    Plasmid

    #99240

    Purpose

    Type II Hexokinase expressed in AS-30D rat hepatoma cell line

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 1997 Sep 5;272(36):22776-80

    Insert

    Hexokinase II (Hk2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Four silent mutations; P (115)L, A (192) V, F (78…

    Promoter

    CMV I.E.

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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