2,294 results

1. p130Cas Y15F

   Plasmid

   #186154

   Purpose

   For expressing recombinant p130Cas in insect cells.

   Depositor

   Michael Rosen

   Article

   Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

   Insert

   BCAR1 (Bcar1 Rat)
   

   Tags

   His6 (Tev)

   Expression

   Insect

   Mutation

   15Y residues mutated to Alanine

   Promoter

   polyhedrin

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Gria3-GFP KI

   Plasmid

   #131491

   Purpose

   Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria3, Gria3 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 barr1 HA

   Plasmid

   #14693

   Depositor

   Robert Lefkowitz

   Article

   Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

   Insert

   beta arrestin 1 (Arrb1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 19, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMRX-IP/Venus-rLC3

   Plasmid

   #58740

   Purpose

   Expresses Venus-rLC3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   Venus

   Available Since

   Dec. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pFsynW SYB2 reporter

   Plasmid

   #197276

   Purpose

   Encodes for viral expression of wild type Synaptobrevin 2 (SYB2) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYB2 and HaloTag (Vamp2 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   N/A

   Promoter

   hSyn (human synapsin I promoter)

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

   Plasmid

   #174900

   Purpose

   amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

   Insert

   CRFR1 (Crhr1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A95TAG

   Promoter

   EF1

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-ALK7 T194D

   Plasmid

   #80886

   Purpose

   mammalian expression of ALK7 T194D

   Depositor

   Aristidis Moustakas

   Article

   Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

   Insert

   ALK7 (Acvr1c Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T194D (constitutively active)

   Promoter

   CMV

   Available Since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

8. SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

   Plasmid

   #123615

   Purpose

   CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3(R33A/K34A)-P2A-EGFP
   

   Expression

   Mammalian

   Mutation

   R33A/K34A in rAPOBEC1, D10A in SpCas9

   Promoter

   CAG

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEmerald-Homer1-C-18

   Plasmid

   #54120

   Purpose

   Localization: Membrane Adaptor, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Homer1 (Homer1 Rat)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCMV-dCpf1-eBE

    Plasmid

    #107688

    Purpose

    Expresses dLbCpf1-eBE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-eBE
    

    Expression

    Mammalian

    Mutation

    dLbCpf1(D832A/E925A/D1148A)

    Promoter

    CMV

    Available Since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmTurqoise KIF1A shRNA

    Plasmid

    #102849

    Purpose

    Generates shRNA specific to Rat KIF1A along with cell fill pmTurqoise

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A-shRNA (KIF1A Rat)
    

    Use

    RNAi

    Expression

    Mammalian

    Promoter

    H1-RNA

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-Pak1 83-149

    Plasmid

    #12216

    Depositor

    Jonathan Chernoff

    Article

    Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

    Insert

    Pak1 (Pak1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Dominant negative Pak1: autoinhibitory domain, aa…

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-BE-VQRCas9

    Plasmid

    #101740

    Purpose

    expression of an optimized Cas9 for base-editing in zebrafish

    Depositor

    Shuo Lin

    Article

    Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

    Insert

    rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, VQR mutations

    Promoter

    CMV

    Available Since

    Jan. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HA-Nrxn1α-LNS3

    Plasmid

    #128107

    Purpose

    Express HA tagged Nrxn1α-LNS3

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS3 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-HA-Nrxn1α-LNS1

    Plasmid

    #128105

    Purpose

    Express HA tagged Nrxn1α-LNS1

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nrxn1a-LNS1 (Nrxn1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMV-VGF

    Plasmid

    #107575

    Purpose

    Encodes the entire VGF cDNA sequence under the control of CMV promoter. Allows expression of VGF in mammalian cells.

    Depositor

    Andrea Levi

    Insert

    VGF (Vgf Rat)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF Synaptophysin-GCamp6s

    Plasmid

    #188981

    Purpose

    Presynaptic fluorescent Ca2+ indicator

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Rat, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-hSyn-Galpha-oA ONE-GO

    Plasmid

    #204335

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-oA ONE-GO
    

    Use

    Lentiviral

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid

    #228941

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IP-EGFP-LOV2-Cyb5a

    Plasmid

    #233348

    Purpose

    Stable expression of EGFP-LOV2(N538E)-Cyb5a in mammalian cells. This construct lacks BAX domain, and causes no rupture upon photostimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus is deleted (Cyb5a 95-134) and N538E

    Available Since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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