We narrowed to 6,267 results for: pcDNA 3.1

Showing: 991 - 1020 of 6267 results

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pcDNA3.1(+)-FluoSTEP-AktAR

Plasmid

#181856

Purpose

Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AktAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Firefly-ccdB

Plasmid

#87069

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsMex3B_AD

Plasmid

#148478

Purpose

Mammalian Expression of HsMex3B

Depositor

Elisa Izaurralde

Insert

HsMex3B (MEX3B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-aDTX-lumitoxin

Plasmid

#51686

Purpose

Expression vector for aDTX-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

aDTX-lumitoxin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.7 (pcDNA3.1+)

Plasmid

#75192

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1delta

Plasmid

#124142

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pY117 (pcDNA3.1-huMb3Cpf1)

Plasmid

#92293

Purpose

Expression of humanized Mb3Cpf1

Depositor

Feng Zhang

Insert

huMb3Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-PITPalpha

Plasmid

#58277

Purpose

mammalian expression of human PITP alpha

Depositor

Shamshad Cockcroft

Insert

PITP alpha (PITPNA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsMex3C_AD

Plasmid

#148481

Purpose

Mammalian Expression of HsMex3C

Depositor

Elisa Izaurralde

Insert

HsMex3C (MEX3C Human)

Expression

Mammalian

Mutation

L11P in Mex3C

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_LN-HA-AGO2-WT

Plasmid

#92009

Purpose

Expresses Lambda-N-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

Plasmid

#159132

Purpose

Expresses BACH1 in mammalian cells

Depositor

Michele Pagano

Article

Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

Insert

BACH1 (BACH1 Human)

Tags

2X-FLAG _ 2X-STREP

Expression

Mammalian

Mutation

Y11F

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-TS3

Plasmid

#104353

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Mutation

AA1-733 ITGB2 present

Promoter

CMV

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hFluc-V5-6xbsMS2_U

Plasmid

#147639

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Insert

6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-TTF-1-HDD

Plasmid

#52063

Purpose

It encodes a mutant of TTF-1 in which the homeodomain is deleted

Depositor

David Mu

Article

Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

Insert

Thyroid Transcription Factor 1 (NKX2-1 Human)

Expression

Mammalian

Mutation

Homeodomain deleted

Available Since

April 11, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7A-mGFP

Plasmid

#62633

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-K650E

Plasmid

#191757

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1948A>G (p.K650E) + silent mutation c.1938C&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-) Hu 4-1BB(CD137)-Fc

Plasmid

#183251

Purpose

Soluble expression of human 4-1BB (CD137)-Fc. Human IgG1 Fc.

Depositor

Alexander McLellan

Insert

41BB Fc

Expression

Mammalian

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX3 (pcDNA3.1+)

Plasmid

#75204

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (Slc24a3 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-600n

Plasmid

#13541

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E WT

Tags

ECFP and Venus

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsUNR_AA

Plasmid

#148239

Purpose

Mammalian Expression of HsUNR

Depositor

Elisa Izaurralde

Insert

HsUNR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_Granulin 2+linker3

Plasmid

#176920

Purpose

express Granulin 2 with linker 3 in mammalian cells

Depositor

Thomas Kukar

Article

Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

Insert

Granulin 2+linker3 (GRN Human)

Tags

Twin-Strep and FLAG tags encoded after Signal Pep…

Expression

Mammalian

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 FLIPglu-170nDelta13V

Plasmid

#18018

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pY120 (pcDNA3.1-huFbCpf1)

Plasmid

#92296

Purpose

Expression of humanized FbCpf1

Depositor

Feng Zhang

Insert

huFbCpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.3 (pcDNA3.1+)

Plasmid

#75191

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-6His-MyoX

Plasmid

#47607

Depositor

Emanuel Strehler

Article

Bennett et al Biochem Biophys Res Commun. 2008 May 2;3

Insert

MyoX (MYO10 Human)

Expression

Mammalian

Mutation

Q680R

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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