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Showing: 1321 - 1360 of 4899 results

  1. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

    Plasmid
    #42335
    Purpose
    A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Has Service
    Cloning Grade DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    Available since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    42335-plasmid-map-sequence-id-275689

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  8. pAcCas12nHS_RV6_empty

    Plasmid
    #203811
    Purpose
    Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.
    Depositor
    Quanjiang Ji
    Article
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Insert
    AcCas12nHS_RV6
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Aug. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    203811-plasmid-map-sequence-id-405075

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  9. pMO746

    Plasmid
    #117480
    Purpose
    upp in an artificial operon with npt from pMO9071 and AmpR-pUC ori from pCR4/TOPO, Pnpt-npt-upp;, KanR
    Depositor
    Judy Wall
    Article
    Parks et al Science. 2013 Mar 15;339(6125):1332-5. d
    Insert
    uracil phosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    117480-plasmid-map-sequence-id-273029

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  11. pAAV-flex-GFP-shRNA-scramble

    Plasmid
    #182503
    Purpose
    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182503-plasmid-map-sequence-id-355964

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  18. pAAV-iCre-2A-venus

    Plasmid
    #182499
    Purpose
    Expresses iCre-venus. The promoter is a fragment of the hdc gene promoter that gives pan neuronal expression
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    iCre-2A-Venus
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182499-plasmid-map-sequence-id-355960

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  21. pAAV-CMV-dSa VP64 Hbb

    Plasmid
    #99693
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99693-plasmid-map-sequence-id-194561

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  22. pTA1cI-T3

    Plasmid
    #193789
    Purpose
    Expresses truncated version of lambda CI named T3 under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Truncated version of Lambda CI, named T3, where the first two codons are deleted
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193789-plasmid-map-sequence-id-389812

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  23. KG#797

    Plasmid
    #110916
    Purpose
    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Use
    Tags
    GFP (contains 3 artificial introns)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110916-plasmid-map-sequence-id-212580

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  24. KG#920

    Plasmid
    #110937
    Purpose
    Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:
    Inserts
    mig-13 promoter
    INS-22-Emerald
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110937-plasmid-map-sequence-id-212471

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  34. Bxb1_AJMA

    Plasmid
    #183760
    Purpose
    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003
    Inserts
    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    Use
    Tags
    Lck and NES
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    183760-plasmid-map-sequence-id-460683

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  37. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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  38. KG#203

    Plasmid
    #110880
    Purpose
    Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1
    Inserts
    rab-3 promoter
    pde-4d (isoform d) cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110880-plasmid-map-sequence-id-212521

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  40. pYL236

    Plasmid
    #173184
    Purpose
    CRISPR donor plasmid for making the WT EZH2 control cell line. It contains a puromycin resistance gene and an mCherry gene
    Depositor
    Thomas Cech
    Article
    Long et al Nat Genet. 2020 Sep;52(9):931-938. doi:
    Insert
    EZH2 (EZH2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    173184-plasmid-map-sequence-id-341220

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Showing: 1321 - 1360 of 4899 results