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Plasmid#214632Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0424
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0425-luc2
Plasmid#214633Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0425
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0395-luc2
Plasmid#214627Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0395
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0402-luc2
Plasmid#214629Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0402
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0373-luc2
Plasmid#214617Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0373
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0391-luc2
Plasmid#214626Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0391
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0403-luc2
Plasmid#214630Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0403
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0404-luc2
Plasmid#214631Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0404
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0389-luc2
Plasmid#214625Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0389
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0388-luc2
Plasmid#214624Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0388
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_10xUAS-CMVmin-BC0401-luc2
Plasmid#214628Purposebarcoded assay, UAS sensor for split TEV assaysDepositorInsert10x clustered UAS element with barcode 0401
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-msEGFP1.8
Plasmid#208619PurposeMammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.DepositorInsertmsEGFP1.8
UseTagsExpressionMammalianMutation(compared with pEGFP-C1, protein level) S30R, Y39…PromoterCMVAvailable sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDHM1_ARATH
Plasmid#204236PurposeBacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDHM1_ARATH (mMDH1 Mustard Weed)
UseTags6X HisExpressionBacterialMutationDoes done include the first 52 residues that are …PromoterLacAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL45
Plasmid#174067PurposeExpresses CIB1 in S. cerevisiae. Includes inducible lac operon.DepositorInsertpGal1-LacO-3NLS-CIB1-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH126
Plasmid#172147PurposeMicroscopy inclusion body assayDepositorInsertpCDF.SUMO-B.(SD4).CD
UseTagsExpressionBacterialMutationPylBCD operon (SUMO tag inserted for PylB; RBS in…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXF90
Plasmid#139044PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag::HDEL (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY131
Plasmid#130956PurposeEngineered sgRNA-LEB2 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAnderson promoter J23100Available sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY76
Plasmid#130963PurposeEngineered sgRNA-LEA2 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY74
Plasmid#130945PurposeEngineered sgRNA-G6 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-G6
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY111
Plasmid#130942PurposeEngineered sgRNA-LEB4 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY110
Plasmid#130940PurposeEngineered sgRNA-LEB3 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY77
Plasmid#130964PurposeEngineered sgRNA-LEB2 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY124
Plasmid#130946PurposeEngineered sgRNA-G6-2MS2 (2 MS2 aptamers) generator circuit including promoter PbadDepositorInsertsgRNA-G6-2MS2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPbadAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY112
Plasmid#130944PurposeEngineered sgRNA-LEB5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEB5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY107
Plasmid#130943PurposeEngineered sgRNA-LEA5 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA5
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY105
Plasmid#130939PurposeEngineered sgRNA-LEA3 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY106
Plasmid#130941PurposeEngineered sgRNA-LEA4 (2 BoxB aptamers) generator circuit including promoter Plux2DepositorInsertsgRNA-LEA4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPlux2Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGeneSwitch
Plasmid#125216PurposeArtificial phase 2 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL80
Plasmid#125219PurposeArtificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL80
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(1)_T2AGeneSwitch
Plasmid#125215PurposeArtificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGeneSwitch
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
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GLRX3
Plasmid#39036PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGLRX3 (GLRX3 Human)
UseTagsHis TevExpressionBacterialMutationA PCR artifact adds a C-terminal sequence includi…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG-deltaA
Plasmid#33151DepositorInsertRP51A-synthetic minimal intron (RPS17A Budding Yeast)
UseTagsLacZExpressionYeastMutationThe RP51A sequence was modified by digestion with…PromoterAvailable sinceDec. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFloxin-Ofd1-Myc-S437R
Plasmid#24577DepositorInsertOfd1 (Ofd1 Mouse)
UseCre/Lox and Mouse TargetingTagsMycExpressionMutationSerine 437 changed to Arginine (codon AGT to CGT)…PromoterAvailable sinceMay 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
JD639
Plasmid#160398PurposeBinary vector pGWB14 including miR396-resistant Vitis GRF4-GIF1 under 35S promoter/Plant transformationDepositorInsert35S::vitis miR396-resistant GRF4-GIF1 chimera
UseBinary vectorTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
JD690
Plasmid#160397PurposeBinary vector pGWB14 including citrus GRF4-GIF1 under 35S promoter/ Plant transformationDepositorInsert35S::citrus GRF4-GIF1 chimera
UseTagsExpressionMutationPromoter35SAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A-APEX2-ChBP
Plasmid#108879PurposeExpression of APEX2 fused to a nanobody against mCherry (includes green cytoplasmic reporter)DepositorInsertEGFP-P2A-APEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP703
Plasmid#80001PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP703DepositorInserthistone cluster 1 H2B family member j (H2BC11 Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pR26 GFP Dest
Plasmid#74283PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV2
Plasmid#180516PurposeAgrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and apolylinkerDepositorInsertTobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
UseTagsExpressionPlantMutationPromoter35S CaMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-Cherry-sgRNA
Plasmid#221387Purposeepisomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAsDepositorInsertnone, cloning vector for introduction of targeting sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-SUV39H2-FL
Plasmid#89135Purposemammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase from pSG5, includes 5' and 3' noncoding regionsDepositorInsertSUV39H2 (SUV39H2 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTara:500
Plasmid#60717PurposeExpresses T7 RNAP from the arabinose inducible promoter PBAD with a RBS strength of 500 from the Salis RBS calculator. Also includes a constitutive araC ORFDepositorInsertT7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP-CAAX
Plasmid#223558PurposePlasmid expressing YFP(Venus) followed by the last 25 amino acids of human KRas4b including the polybasic regions and CAAX prenylation boxDepositorInsertYFP(Venus) - KRas4b
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pR26 iBFP Dest
Plasmid#74284PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloningDepositorInsertRosa26 5-homology region
UseTagsExpressionMutationPromoterAvailable sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-loop
Plasmid#132695PurposepRDV5 plasmid containing a loop Sybody for the generation of a qPCR standard curveDepositorInsertSb_loop including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD103-ARG1
Plasmid#106475PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimuriumDepositorInsertARG1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLuxAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only