Skip to main content
Addgene
Showing: 1651 - 1700 of 18426 results
  1. pLenti-C-Myc-DDK-IRES-Puro METTL3

    Plasmid
    #200724
    Purpose
    Lentiviral expression of Flag-Tagged human METTL3
    Depositor
    Insert
    METTL3 (METTL3 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. LO601: pMVP (R4-R3) destabilized firefly luciferase (Luc2P)

    Plasmid
    #132926
    Purpose
    pMVP R4-R3 entry plasmid, contains destabilized Firefly Luciferase (Luc2P) for 3- or 4-component MultiSite Gateway Pro assembly
    Insert
    Luc2P
    Use
    Luciferase and Synthetic Biology; Pmvp gateway en…
    Tags
    PEST destabilization domain
    Expression
    Mutation
    Promoter
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid
    #219964
    Purpose
    Express human BCR-ABL1-P190 in mammalian cells
    Depositor
    Insert
    BCR-ABL1-P190 (ABL1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69545
    Purpose
    Tet-On system expressing GFP tagged with 2x nuclear localization signals.
    Depositor
    Insert
    eGFP
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pUbC-OsTIR1-myc-IRES-scFv-sfGFP

    Plasmid
    #84563
    Purpose
    Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP
    Depositor
    Insert
    Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
    Use
    Lentiviral
    Tags
    myc tag (9x)
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid
    #102886
    Purpose
    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Insert
    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    CAG
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine

    Plasmid
    #50454
    Purpose
    Double floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Has Service
    AAV8
    Insert
    hM3D(Gq)-IRES-mCitrine (CHRM3 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid
    #200726
    Purpose
    Lentiviral expression of Flag-Tagged human HEXIM1
    Depositor
    Insert
    HEXIM1 (HEXIM1 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV-CMV-rtTA-IRES-GFP-WPRE-pA

    Plasmid
    #183709
    Purpose
    AAV designed to express rtTA and eGFP from a CMV promoter
    Depositor
    Insert
    rtTA
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Has Service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Expression
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pLenti-C-Myc-DDK-IRES-Puro HNRNPA1

    Plasmid
    #200727
    Purpose
    Lentiviral expression of Flag-Tagged human HNRNPA1
    Depositor
    Insert
    HNRNPA1 (HNRNPA1 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  13. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid
    #193298
    Purpose
    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs
    Depositor
    Insert
    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 1651 - 1700 of 18426 results