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Flag-FOXB1

Plasmid

#153112

Purpose

Expression of Flag tagged human FOXB1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXB1 (FOXB1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSEED-OLLAS:HA

Plasmid

#219647

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

OLLAS:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Htb2-GFP

Plasmid

#117669

Purpose

Colorize the nuclei

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

Htb2-GFP

Expression

Yeast

Mutation

Contains the gene encoding the Histone Htb2 from …

Promoter

pGAP

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4

Plasmid

#153119

Purpose

Expression of Flag tagged human FOXD4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4 (FOXD4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean-T-SpyTag

Plasmid

#243787

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCerulean from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCerulean

Tags

6x His tag

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-S-SpyTag

Plasmid

#243786

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCherry from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCherry

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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TVMV Protease-mCherry

Plasmid

#243794

Purpose

Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A-SpyTag

Plasmid

#243769

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S-SpyTag

Plasmid

#243770

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-T-SpyTag

Plasmid

#243771

Purpose

Expresses multifunctional construct enabling the YES-gated release of mGreenLantern from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR S-SpyTag

Plasmid

#243772

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR sortase eSrtA(4S9) treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A OR T-SpyTag

Plasmid

#243773

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-S OR T-SpyTag

Plasmid

#243774

Purpose

Expresses multifunctional construct enabling the OR-gated release of mGreenLantern from materials in response to sortase eSrtA(4S9) OR TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mGreenLantern-A AND T-SpyTag

Plasmid

#243776

Purpose

Expresses multifunctional construct enabling the AND-gated release of mGreenLantern from materials in response to sortase eSrtA(2A9) AND TEV treatment. Contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mGreenLantern

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-EYFP:HA

Plasmid

#219656

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

EYFP:HA SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

Plasmid

#147555

Purpose

Mammalian Expression of HsSmg5-Q123E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-Q123E (SMG5 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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KHBD00280

Plasmid

#34407

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7230 (rib Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only