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Showing: 661 - 680 of 2333 results

  9. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Article
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    99377-plasmid-map-sequence-id-191028

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  10. FcD265A-FLAG

    Plasmid
    #65420
    Purpose
    Mammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 production
    Depositor
    Dane Wittrup
    Article
    Zhu et al Cancer Cell. 2015 Apr 13;27(4):489-501.
    Insert
    FcD265A-FLAG
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D265A
    Promoter
    Modified CMV Promoter
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51761-plasmid-map-sequence-id-79377

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  20. lentiCas9-Venus

    Plasmid
    #70267
    Purpose
    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    Cas9
    Venus Reporter
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS and P2A
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70267-plasmid-map-sequence-id-126420

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Showing: 661 - 680 of 2333 results