We narrowed to 830 results for: Hr

1. Flag-hPKL S113A

   Plasmid

   #107160

   Purpose

   For mammalian expression of Flag-hPKL S113A

   Depositor

   Jesse Rinehart

   Article

   Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

   Insert

   Flag-hPKL S113A (PKLR Human)
   

   Use

   Retroviral

   Tags

   N-term Flag

   Expression

   Mammalian

   Mutation

   Serine 113 mutated to alanine

   Promoter

   CMV

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO shLuc/FLAG Metap2

   Plasmid

   #209316

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 Human, human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO shLYCHOS/LYCHOS WT-FLAG

   Plasmid

   #209315

   Purpose

   Lentiviral construct expressing shRNA against LYCHOS and LYCHOS WT-FLAG (resistant to shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO shLYCHOS/FLAG Metap2

   Plasmid

   #209314

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28 mOct4-POU

   Plasmid

   #206393

   Purpose

   Expresses the DNA binding domain of the Oct4 with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   Oct4 (Pou5f1 Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB-CG-LYCHOS WT-HA-GFP

   Plasmid

   #199687

   Purpose

   Expression of LYCHOS WT in insect cells

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   LYCHOS (GPR155 Human)
   

   Use

   Tags

   10xHis, EGFP, HA, HRV 3C, and His

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-LYCHOS (GPR155) ∆DEP-HA

   Plasmid

   #199683

   Purpose

   Transient expression of LYCHOS (GPR155) ∆DEP-HA

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   DEP domain deleted

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5 LYCHOS (GPR155) Y551A-HA

   Plasmid

   #199676

   Purpose

   Transient expression of LYCHOS (GPR155) Y551A mutant

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   GPR155 (GPR155 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y551 mutated to A

   Promoter

   CMV

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a LZ LED WT-FLAG

   Plasmid

   #199679

   Purpose

   Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper LYCHOS LED WT (GPR155 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-LYCHOS (GPR155) P44A-HA

    Plasmid

    #199675

    Purpose

    Transient expression of LYCHOS (GPR155) P44A mutant

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P44 mutated to A

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5-LYCHOS (GPR155) E48Q-HA

    Plasmid

    #199662

    Purpose

    Transient expression of LYCHOS (GPR155) E48Q mutant

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E48 mutated to Q

    Promoter

    CMV

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRK5-LYCHOS (GPR155) F43I-HA

    Plasmid

    #199674

    Purpose

    Transient expression of LYCHOS (GPR155) F43I mutant

    Depositor

    Roberto Zoncu

    Article

    Shin et al Science. 2022 Sep 16;377(6612):1290-1298

    Insert

    GPR155 (GPR155 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F43 mutated to I

    Promoter

    CMV

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. MBP-rPKL S12TAG

    Plasmid

    #107155

    Purpose

    MBP-rPKL construct for production and purification of rat PKL pS12

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 12 to TAG for phosphoserine insertion

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. MBP-rPKL S113TAG

    Plasmid

    #107157

    Purpose

    MBP-rPKL S113TAG for production and purification of rat PKL pS113

    Depositor

    Jesse Rinehart

    Article

    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

    Insert

    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    

    Use

    Tags

    C-term 6-his and N-term MBP

    Expression

    Bacterial

    Mutation

    Serine 113 changed to TAG for phosphoserine inser…

    Promoter

    PBAD

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SF-SON IRES Puro

    Plasmid

    #214402

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. SF-SON IRES Tomato

    Plasmid

    #214403

    Purpose

    Expresses human SOX10, OLIG2, NKX6.2 and tdTomato

    Depositor

    Tanja Kuhlmann

    Article

    Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LSL-Cas9-Rosa26TV

    Plasmid

    #61408

    Purpose

    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    3xFLAG and P2A

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CAG

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60228

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV:ITR-U6-sgRNA(NeuN)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60227

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA targeting the mouse gene NeuN. AAV backbone.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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