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We narrowed to 4,810 results for: codon optimized

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Showing: 661 - 680 of 4810 results

  1. pBHM2648

    Plasmid
    #229990
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229990-plasmid-map-sequence-id-461146

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  2. pBHM2649

    Plasmid
    #229991
    Purpose
    Carries OsTIR1-t2a-mNG-mIAA7, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mIAA7_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229991-plasmid-map-sequence-id-461155

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  5. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213027
    Purpose
    Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    D702Y (RT- reverse transcriptase catalytic dead)
    Promoter
    CMV
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213027-plasmid-map-sequence-id-452358

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  7. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Richard Murray
    Article
    Kapasiawala et al ACS Synth Biol. 2024 Dec 20;13(12):3976-
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227645-plasmid-map-sequence-id-452394

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  9. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  10. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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  16. pCW57-GFP-P2A-EcASPase

    Plasmid
    #252079
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of E.coli derived aspartate lyase in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Lydia Finley
    Article
    Brunner et al Mol Cell. 2026 Feb 26:S1097-2765(26)0009
    Insert
    Codon optimized E.coli derived aspartate ammonia-lyase
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Expression
    Mammalian
    Mutation
    Codon optimized
    Promoter
    TRE
    Available Since
    March 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    252079-plasmid-map-sequence-id-501997

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  17. pBHM2643

    Plasmid
    #229527
    Purpose
    Carries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
    Depositor
    Hiten Madhani
    Insert
    OsTIR1
    Tags
    t2a-mNeonGreen-mAID_opt
    Expression
    Bacterial and Yeast
    Mutation
    F74G
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    229527-plasmid-map-sequence-id-459548

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  20. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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Showing: 661 - 680 of 4810 results