We narrowed to 5,798 results for: pcas

1. pCAG-ATG14

   Plasmid

   #203565

   Purpose

   Mammalian expression of ATG14

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG14 (ATG14 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL2621 (pCas8_Vch)

   Plasmid

   #200893

   Purpose

   Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas8
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL2623 (pCas6_Vch)

   Plasmid

   #200895

   Purpose

   Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas6
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9-VRER_2A_GFP

   Plasmid

   #75475

   Purpose

   Cas9 VRER variant that detects NGCG PAM, combined with 2A_GFP for expression control

   Depositor

   Marc Tessier-Lavigne

   Article

   Paquet et al Nature. 2016 May 5;533(7601):125-9. doi:

   Insert

   Cas9-VRER
   

   Use

   CRISPR

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335E, T1337R

   Promoter

   CAG

   Available Since

   May 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-B19M

   Plasmid

   #59923

   Purpose

   Expression vector for Rabies virus SAD B19 matrix protein gene

   Depositor

   Ian Wickersham

   Insert

   Rabies virus matrix protein
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-EnvARGCD

   Plasmid

   #100800

   Purpose

   express fusion protein of EnvA (ASLV-A envelope protein) extracellular and transmembrane domains with rabies virus SAD B19 strain glycoprotein intracellular domain

   Depositor

   Ian Wickersham

   Insert

   fusion protein of EnvA extracellular and transmembrane domain with rabies virus glycoprotein intracellular domain
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-tdTHC

   Plasmid

   #112621

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BtdTomato_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-tdTomato (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-6020nes

   Plasmid

   #108656

   Purpose

   A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

   Depositor

   Michiyuki Matsuda

   Article

   Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

   Insert

   hyBRET-JNK-EV
   

   Tags

   XFP

   Expression

   Mammalian

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-GFPmG1

   Plasmid

   #50444

   Purpose

   Expression of a EGFP variant in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GFPmG1
   

   Expression

   Mammalian

   Mutation

   E143K, N147Q

   Promoter

   CAG

   Available Since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. eSpCas9-EF-5Pou5f1

    Plasmid

    #174872

    Purpose

    CRISPR vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Pou5f1 (Pou5f1 Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. LLP784_SnoopCatcher-DNMT3A-CO

    Plasmid

    #211777

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to DNA methyltransferase DNMT3A, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Spy-EZH2
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect EZH2 e…

    Promoter

    pEF1a and pSV40

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas_5+7_gfp

    Plasmid

    #60595

    Purpose

    encodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Cas_5+7_gfp
    

    Expression

    Bacterial

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-ROSAf

    Plasmid

    #54323

    Purpose

    TALEN expression construct for targeting the murine ROSA26 locus. It recognize the sequence: CTGCAACTCCAGTCT. Can be used with the pBS SK mCherryROSAbsr plasmid

    Depositor

    Silvestro Conticello

    Insert

    TALEN for the murine ROSA26 locus
    

    Use

    Mouse Targeting and TALEN

    Expression

    Mammalian

    Promoter

    CAGG

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-VHC

    Plasmid

    #112618

    Purpose

    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Venus (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Tags

    Venus

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGGS-AcGFP-C

    Plasmid

    #203325

    Purpose

    Express N-terminal AcGFP-fused protein in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    AcGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCaRNA3-eIFiso4E120(+)

    Plasmid

    #86092

    Purpose

    This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

    Depositor

    Aiming Wang

    Article

    Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

    Insert

    eIFiso4E
    

    Expression

    Plant

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-Bmp2

    Plasmid

    #163587

    Purpose

    Expresses BMP-2 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmp2 (Bmp2 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMA-SpCas9-g9

    Plasmid

    #80792

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMA-SpCas9-g8

    Plasmid

    #80791

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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