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We narrowed to 1,752 results for: pyogenes Cas9

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Showing: 661 - 680 of 1752 results

  8. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available Since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    61408-plasmid-map-sequence-id-333613

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  9. lenti-EF1a-dCas9-VPR-eGFP

    Plasmid
    #241307
    Purpose
    3rd generation lenti vector encoding dCas9 (s. Pyogenes) fused with the VP64-p65-Rta(VPR) and a 2A GFP fluorescence marker
    Depositor
    Robby Bowles
    Insert
    eGFP
    Use
    Lentiviral
    Available Since
    Nov. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241307-plasmid-map-sequence-id-483039

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  10. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  13. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid
    #71829
    Purpose
    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    BACH2-sgRNA8 (BACH2 Synthetic, S. pyogenes, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E756A inactiva…
    Promoter
    U6
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71829-plasmid-map-sequence-id-131024

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  15. dCas9-CBP RING-PHD-HAT

    Plasmid
    #179549
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179549-plasmid-map-sequence-id-354747

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  16. dCas9-p300 RING-PHD-HAT

    Plasmid
    #179546
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    179546-plasmid-map-sequence-id-354744

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  19. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

    Plasmid
    #62373
    Purpose
    Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells
    Depositor
    David Liu
    Article
    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do
    Insert
    (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
    Tags
    (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…
    Expression
    Bacterial
    Mutation
    Y67S amino acid substitution in (-30)GFP renders …
    Promoter
    T7
    Available Since
    June 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    62373-plasmid-map-sequence-id-263816

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  20. pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)

    Plasmid
    #64217
    Purpose
    Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
    Depositor
    Ralf Kuehn
    Article
    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103
    Inserts
    Cas9
    hU6 promoter; BbsI sites for sgRNA
    H1 promoter; BamHI site for shRNA
    Use
    CRISPR
    Tags
    3xFLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh, H1, and U6
    Available Since
    Sept. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    64217-plasmid-map-sequence-id-123057

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Showing: 661 - 680 of 1752 results