We narrowed to 10,419 results for: plasmids 101

1. pAAV-FLEX-SaCas9-U6-sgSlc17a6

   Plasmid

   #124847

   Purpose

   Mutagenesis of Slc17a6 with SauCas9

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a6 gRNA (Slc17a6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Promoter

   hEF-1a

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

   Plasmid

   #205766

   Purpose

   Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells

   Depositor

   Edward Boyden

   Article

   Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

   Insert

   JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. gh92

   Plasmid

   #106763

   Purpose

   expression of gRNA targeting FUT8

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   FUT8 (FUT8 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-UBC-mCD24-T2A-GFP

   Plasmid

   #205448

   Purpose

   lentiviral plasmid for expression of mouse CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   mCD24 (Cd24a Mouse)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. miniCGBE1 (pBM1219)

   Plasmid

   #140253

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLOVE-Nmyc

   Plasmid

   #15951

   Depositor

   Miguel Ramalho-Santos

   Article

   Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

   Insert

   v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   The recombination reaction required to clone &quo…

   Available Since

   Nov. 7, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pJBEI-6410

   Plasmid

   #47049

   Purpose

   BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli

   Depositor

   Taek Soon Lee

   Article

   Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

   Insert

   Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
   

   Use

   Synthetic Biology; Bglbrick

   Expression

   Bacterial

   Promoter

   PlacUV5

   Available Since

   Sept. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.26-Ccnd2PromoterRegion2(0.5kb)

   Plasmid

   #228059

   Purpose

   0.5 kb from mouse Ccnd2 promoter in front of firefly luciferase reporter

   Depositor

   Kenneth Chen

   Article

   Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

   Insert

   Ccnd2 promoter (Ccnd2 Mouse)
   

   Use

   Luciferase

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.26-Igf2Promoter

    Plasmid

    #228060

    Purpose

    0.9 kb from mouse Igf2 promoter in front of firefly luciferase reporter

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Igf2 promoter (Igf2 Mouse)
    

    Use

    Luciferase

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-SaCas9-U6-sgGrin1

    Plasmid

    #124852

    Purpose

    Mutagenesis of Grin1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin1 (Grin1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-SYP1-miniSOG-citrine

    Plasmid

    #50971

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Insert

    Synaptophysin-miniSOG-citrine (Syp Rat)
    

    Use

    AAV

    Tags

    citrine and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

    Plasmid

    #214733

    Purpose

    Expresses mCherry protein in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA (scrambled) / mCherry
    

    Use

    AAV

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Has Service

    AAV8

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBR_attB(bxb)_ccdB_lox

    Plasmid

    #183763

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ccdB
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LLP469 pEF1a-mCherry-EMPTY-gRNA

    Plasmid

    #100958

    Purpose

    mCherry driven by EF1a, empty gRNA scaffold driven by U6

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    gRNA empty backbone with RFP
    

    Use

    CRISPR

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

    Plasmid

    #216531

    Purpose

    Targeting vector for CRISPRd generation

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    ABI-dCas9-mCherry
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Tags

    Lck and NES

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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