161,273 results

1. pBAD-LSSmCherry1

   Plasmid

   #89986

   Purpose

   Long stokes-shift variant of red fluorescent protein mCherry

   Depositor

   Robert Campbell

   Article

   Shen et al PLoS One. 2017 Feb 27;12(2):e0171257. do

   Insert

   LSSmCherry1
   

   Use

   Tags

   6xHis-T7-Xpress

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPAICS-EGFP

   Plasmid

   #99108

   Purpose

   Expresses human PAICS-EGFP fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   PAICS (PAICS Human)
   

   Use

   Tags

   EGFP (enhanced green fluorescent protein)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. mVenus-pBAD

   Plasmid

   #54845

   Purpose

   Localization: Protein Expression Vector , Excitation: 515, Emission: 528

   Depositor

   Atsushi Miyawaki, Michael Davidson

   Article

   Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mVenus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCAG-TetON-3G

   Plasmid

   #96963

   Purpose

   Mammalian expression of Clontech rtTA driven by human beta-actin promoter

   Depositor

   Elena Cattaneo

   Article

   Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMAL-AGR2

   Plasmid

   #18097

   Depositor

   Ronny Drapkin

   Insert

   AGR2 (AGR2 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

6. ATGL*-V5-APEX2

   Plasmid

   #170574

   Purpose

   A C-terminal APEX2 fusion to the human ATGL S47A protein

   Depositor

   James Olzmann

   Article

   Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

   Insert

   ATGL* (PNPLA2 Human)
   

   Use

   Lentiviral

   Tags

   V5-APEX2

   Expression

   Mammalian

   Mutation

   changed serine 47 to alanine

   Promoter

   CMV

   Available since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-dCas9-FLp300

   Plasmid

   #61356

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH0001

   Plasmid

   #85969

   Purpose

   UCOE-SFFV-dCas9-BFP-KRAB

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   BFP-KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   SFFV

   Available since

   Feb. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pV1093:SpRY

   Plasmid

   #172188

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-SADB19G

    Plasmid

    #32633

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    SADB19G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. yHS1-M7A,H102A

    Plasmid

    #159152

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A,H102A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated both Met 7 and His 102 of the cyt b562 mo…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGEX6P1-HA-MEK1

    Plasmid

    #53159

    Purpose

    Expresses GST-HA tagged human MEK1 from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-Nanog

    Plasmid

    #13354

    Purpose

    Retroviral expression of mouse Nanog

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdCas9

    Plasmid

    #46569

    Purpose

    Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSFFV_Gal4-VP64-IRES-mCherry (pLZA139)

    Plasmid

    #203909

    Purpose

    Encodes wild type Gal4-VP64 transcription factor in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4-VP64-IRES-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAD43-daf-16 RNAi

    Plasmid

    #34833

    Depositor

    Cynthia Kenyon

    Article

    Dillin et al Science. 2002 Oct 25;298(5594):830-4.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-FLEXfrt-SaCas9-U6-sgRNA

    Plasmid

    #124845

    Purpose

    Vector for FLP-dependent expression of SaCas9 with gRNA

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SauCas9 gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLM-CMV-R-Cre

    Plasmid

    #27546

    Purpose

    Lentiviral bicistronic co-expression of Cre and mCherry; linked by a P2A peptide.

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Nat Biotechnol. 2011 Jan;29(1):73-8. doi

    Insert

    mCherry_P2A_Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IDG_CACNG8_OE_1

    Plasmid

    #161698

    Purpose

    Overexpresses CACNG8

    Depositor

    Mike McManus

    Insert

    CACNG8 (CACNG8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTarget_CAST

    Plasmid

    #127926

    Purpose

    Target plasmid containing PSP1

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

    Insert

    PSP1 target (GGTT PAM)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Lenti-CMV-PRB

    Plasmid

    #206165

    Purpose

    pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    Progesteron receptor B (PGR Human)
    

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pColE1_sgRNA_pos8_AmpR

    Plasmid

    #119151

    Purpose

    Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 8 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sfGFP-pBAD

    Plasmid

    #54519

    Purpose

    Localization: Protein Expression Vector , Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    6xHis and SfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. G13-CASE

    Plasmid

    #168127

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA13, GNB3, GNG9 (GNA13, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F125/D126 within GNA13

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. V5-LOV-Turbo-NES

    Plasmid

    #199545

    Purpose

    expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NES and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-tTRKRAB-red

    Plasmid

    #12250

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    TetR-KRAB fusion and dsRED
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    dsRed added to backbone.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Mammalian Pol I

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-Rab21-Q76L (CA)

    Plasmid

    #83422

    Purpose

    Mouse Rab21 mutant that has defects in GTP hydrolysis (GTP-locked, constitutively active). Generated from pEGFP-Rab21 by site-directed mutagenesis.

    Depositor

    Johanna Ivaska

    Article

    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

    Insert

    Rab21 (Rab21 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Residue 76 from Q to L (Q76L: CAG -> CTG)

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDMS151A_Tet-On-3G

    Plasmid

    #191577

    Purpose

    ClonTech-Takara's doxycycline-inducible, rtTA transactivator (Tet-On-3G) cloned into a 2nd generation lentiviral transfer plasmid (pGIPZ).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    Tet-On(R) 3G
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Gs(short)-CASE

    Plasmid

    #168124

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG1

    Expression

    Mammalian

    Mutation

    NLuc is inserted at N136/V137 within GNAS

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge