We narrowed to 15,985 results for: grna

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Publication

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Publication

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-SEP-GluA1 TKIT

Plasmid

#169441

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

Plasmid

#159905

Purpose

Mutagenesis of Slc32a1

Depositor

Larry Zweifel

Insert

Slc32a1 (Slc32a1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas9-ScaligD

Plasmid

#125688

Purpose

Gene knockout/in for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spCas9, sgRNA casette, and ScaligD

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-puro-STING-sh5

Plasmid

#127647

Purpose

Knock-down of human STING

Depositor

Nicolas Manel

Publication

Gratia et al J Exp Med. 2019 May 6;216(5):1199-1213.

Insert

STING shRNA (STING1 Human)

Use

Lentiviral

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGreenPuro-shRNA-Stx3S-C4

Plasmid

#99742

Purpose

3rd generation lentiviral vector expresses shRNA against human Stx3S with a GFP reporter.

Depositor

Thomas Weimbs

Publication

Giovannone et al J Biol Chem. 2018 Apr 13;293(15):5478-54

Insert

shRNA against Stx3S

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Scr shRNA

Plasmid

#59299

Purpose

non-targeting shRNA

Depositor

Ulrike Heberlein

Publication

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Scr shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
MSCV FLIPi P2G_Thy1.1 PTEN

Plasmid

#19749

Depositor

Richard Hynes

Publication

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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p426_Cas9_gRNA-R-ARS607c

Plasmid

#87409

Purpose

p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

Depositor

Aindrila Mukhopadhyay

Publication

Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

Insert

Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c

Use

CRISPR

Promoter

ADH1, pTyrosine

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

Plasmid

#79383

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Zhang 2.0 + scaffold

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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CaTCH Dual-sgRNA_sgBC1-A_sgBC1-C

Plasmid

#154196

Purpose

Lentiviral expression plasmid encoding two sgRNAs for targeting of the CaTCH barcode cassette BC1. Expresses Thy1.1 and a Neo selection marker.

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sgRNA-BC1-A, sgRNA-BC1-C

Use

Lentiviral; Catch barcode activation

Expression

Mammalian

Promoter

hU6 and mU6

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_B358c_attB

Plasmid

#182146

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Publication

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ALB_B358c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-gRNA-ALB_DF_A277c_attB

Plasmid

#182145

Purpose

To insert attB attachment site at ALB in human cells via twinPE

Depositor

David Liu

Publication

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Alb_A277c_attB pegRNA

Expression

Mammalian

Promoter

hU6

Available since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX330-AAVS1

Plasmid

#227272

Purpose

Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in.

Depositor

Laurence Pelletier

Publication

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA Targeting AAVS1 (AAVS1 Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCF806-shRen.713

Plasmid

#186712

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

Depositor

Christof Fellmann

Publication

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

shRen.713

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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QPM-sgR (pTaU3)

Plasmid

#140448

Purpose

For sgRNA expression in monocotyledons protoplasts

Depositor

Caixia Gao

Publication

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

TaU3-sgRNA

Use

CRISPR

Expression

Plant

Promoter

TaU3

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro-humanU6-shRNA FASN

Plasmid

#82327

Purpose

used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

Depositor

Elizabeth Stoll

Insert

shRNA to target Fatty Acid Synthase (FASN) (FASN Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLKO.1 Rheb1 shRNA #2

Plasmid

#26626

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Rheb1 shRNA (RHEB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only