We narrowed to 58,550 results for: tra

1. pQE60-mPU.1-ETS

   Plasmid

   #85733

   Purpose

   Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

   Depositor

   Gregory Poon

   Article

   Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

   Insert

   ETS domain of transcription factor PU.1 (Spi1 Mouse)
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2443 - [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159867

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-mMaple3(PATCs(900bp), NLS, no_atg, no stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

   Plasmid

   #159861

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CFF-EGFP

   Plasmid

   #86061

   Purpose

   A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Fibrocystin (Pkhd1 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains luminal and transmembrane domains of CD8…

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TNPO1-shRNA

   Plasmid

   #86082

   Purpose

   A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. kiCAP-AAV-M.Mus.1

   Plasmid

   #196681

   Purpose

   Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   WVLPSGG insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. kiCAP-AAV-MDV1B

   Plasmid

   #196680

   Purpose

   Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.

   Depositor

   Pardis Sabeti

   Article

   Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   KTVGTVY insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30Xa-SBL1(160S)

   Plasmid

   #66872

   Purpose

   Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
   

   Tags

   Histag from pET30Xa-LIC

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Nanog-CreER targeting construct

   Plasmid

   #59720

   Purpose

   Cre-ER knockin targeting construct for mouse endogenous Nanog locus

   Depositor

   Jacob Hanna

   Article

   Maza et al Nat Biotechnol. 2015 Jun 22. doi: 10.103

   Insert

   creERT2
   

   Use

   Mouse Targeting

   Promoter

   mNanog

   Available Since

   Jan. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI syn iGluf

    Plasmid

    #106121

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR E25D variant
    

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Glu 25 to Asp

    Promoter

    synapsin

    Available Since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSL0376 (His-MBP-StrepII-TniQ)

    Plasmid

    #130641

    Purpose

    Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite-StrepII on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO-shcIAP1-B

    Plasmid

    #44131

    Depositor

    William Hahn

    Article

    Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

    Insert

    c-IAP1 (BIRC2 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 10, 2013

    Availability

    Academic Institutions and Nonprofits only

13. JT504: pMVP/SB/eGFP-DEST

    Plasmid

    #121861

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. JT601: pMVP/SB/mCherry-DEST

    Plasmid

    #121862

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ02B2Gm_AE

    Plasmid

    #66063

    Purpose

    MoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    FACS Controls
    

    Use

    Synthetic Biology

    Available Since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. myc-Miro1 (lacking TM)

    Plasmid

    #127613

    Purpose

    Human Miro1 with the transmembrane deleted

    Depositor

    Josef Kittler

    Article

    Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Deletion of transmembrane domain

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-PRMT9 L182A/D183A/I184A/G185A

    Plasmid

    #79676

    Purpose

    mammalian expression of GFP-PRMT9 catalytically inactive LDIG(182-185)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L182A/D183A/I184A/G185A catalytic mutant

    Promoter

    CMV

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

18. GFP-FKBP-TPD52

    Plasmid

    #172455

    Purpose

    Expression of Tumor Protein D52 (TPD52) with N-terminal GFP-FKBP tag

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Tags

    GFP-FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. GST-TPD54

    Plasmid

    #172462

    Purpose

    Bacterial expression of GST-tagged Tumor Protein D52 like 2 (TPD54/TPD52L2)

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. KN302: pMVP/SB/eCFP-DEST

    Plasmid

    #121863

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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