We narrowed to 12,134 results for: cel.2

1. EGFR D770-N771 insNPG

   Plasmid

   #11016

   Purpose

   Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D770-N771 insNPG (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D770-N771 insNPG

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Available Since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pCAGGS-SMS2-TEV-Twin-Strep

   Plasmid

   #202529

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS2 (SGMS2 Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TGA)

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX4T3-HA-ERK2 GOF

   Plasmid

   #53200

   Purpose

   Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK2 (MAPK1 Human)
   

   Tags

   GST and HA

   Expression

   Bacterial

   Mutation

   R67S/D321N

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

5. GFP-TRIM24

   Plasmid

   #65396

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   TRIM24 (TRIM24 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pSynapsin_NLS_GFP_P2A_His6_GluA2

   Plasmid

   #170453

   Purpose

   Expresses histidine tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   Hexa-histidine

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCTIP2-N174

   Plasmid

   #60858

   Purpose

   Expresses CTIP2 under the EF1a promoter

   Depositor

   Andrew Yoo

   Article

   Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

   Insert

   CTIP2 isoform b (Bcl11b Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

   Plasmid

   #170455

   Purpose

   Expresses SNAP tagged GluA2 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   GluA2 (Gria2 Mouse)
   

   Use

   Lentiviral

   Tags

   SNAP

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLex 983-Micu2

   Plasmid

   #50372

   Purpose

   Expression in mammalian cells

   Depositor

   Vamsi Mootha

   Article

   Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

   Insert

   MICU2 (MICU2 Human)
   

   Use

   Lentivirus

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGEX4T3-HA-ERK2 R67S

    Plasmid

    #53201

    Purpose

    Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    R67S

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLentiCas9-T2A-mApple

    Plasmid

    #193780

    Purpose

    Tricistronic expression of Cas9-mApple-BSD in mammalian cells

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    mApple
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    In frame with Cas9

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid

    #113960

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS6-hα2M-H6

    Plasmid

    #128495

    Purpose

    Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (G27-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX4T3-HA-ERK2 D321N

    Plasmid

    #53202

    Purpose

    Expresses GST and HA tagged human ERK2 D321N from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    ERK2 (MAPK1 Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    D321N

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS6-C-hα2M-H6

    Plasmid

    #128497

    Purpose

    Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    A2M (F789-S1468) (A2M Human)
    

    Tags

    His tag (6x)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

    Plasmid

    #111459

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

    Plasmid

    #226101

    Purpose

    HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SIFI degron helices 1 & 2 deleted (Δ61-112)

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MCHR2-DuET

    Plasmid

    #213338

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    MCHR2 (MCHR2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-lambdaN-HA-DmCCR4-4xmut-dsRNAres_R

    Plasmid

    #147425

    Purpose

    Insect Expression of DmCCR4-4xmut-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmCCR4-4xmut-dsRNAres (twin Fly)
    

    Expression

    Insect

    Mutation

    The following variations comprises from our S2 ce…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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