We narrowed to 16,820 results for: IGH@

1. pENTR CMVie-Lifeact-mRuby2 L1-R5

   Plasmid

   #84389

   Purpose

   Multisite Gateway entry vector for expression of mRuby2-tagged Lifeact in cells including neurons

   Depositor

   Ghassan Mouneimne

   Article

   Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

   Insert

   Lifeact
   

   Use

   Gateway

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB425

   Plasmid

   #74819

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETDuet-msGC-NT2

   Plasmid

   #74763

   Purpose

   expresses the N-terminal fragment of manduca sexta soluble guanylyl cyclase subunits alpha and beta

   Depositor

   William Montfort

   Article

   Hu et al J Biol Chem. 2008 Jul 25;283(30):20968-7

   Inserts

   fragment of alfa subunit of sGC
   fragment of beta subunit of sGC
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   fragment 49-471 of the alfa subunit

   Promoter

   T7

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PA-mCherry-miniSOG-MAP4-C-10

   Plasmid

   #57794

   Purpose

   Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   MAP4
   

   Use

   Tags

   PA-mCherry-miniSOG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. miniSOG-MAP4-C-10

   Plasmid

   #57772

   Purpose

   Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

   Depositor

   Michael Davidson

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   MAP4
   

   Use

   Tags

   miniSOG

   Expression

   Mammalian

   Mutation

   G982V and G993C compared to NM_008633.4.

   Promoter

   CMV

   Available since

   Dec. 7, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pHL391

   Plasmid

   #62821

   Purpose

   Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   gfp
   mCherry
   

   Use

   Synthetic Biology

   Tags

   hfp leader, ompC leader, and st7 RBS

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pHL518

   Plasmid

   #62823

   Purpose

   Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   mCherry
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::ompC leader and micC leader

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pHL2021

   Plasmid

   #62833

   Purpose

   Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::sodB leader

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHL2026

   Plasmid

   #62834

   Purpose

   Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

   Depositor

   Han Lim

   Article

   Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

   Inserts

   T710 hfq leader
   gfp
   

   Use

   Synthetic Biology

   Tags

   m9::glmS leader

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 and pLtetO-1

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pHL2027

    Plasmid

    #62835

    Purpose

    Lim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    glmS leader

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pHL2053

    Plasmid

    #62836

    Purpose

    Lim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::ompC leader

    Expression

    Bacterial

    Mutation

    Promoter

    pLlacO-1 and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pHL2073

    Plasmid

    #62837

    Purpose

    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    glmZ leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::glmS

    Expression

    Bacterial

    Mutation

    Promoter

    pConShortNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pHL2075

    Plasmid

    #62838

    Purpose

    Lim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    glmZ leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    glmS

    Expression

    Bacterial

    Mutation

    Promoter

    pConShortNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pHL2085

    Plasmid

    #62840

    Purpose

    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    m8::glmZ leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    glmS

    Expression

    Bacterial

    Mutation

    Promoter

    pConShortNoHind, pLlacO-1, and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pHL2117

    Plasmid

    #62842

    Purpose

    Lim Lab plasmid: AmpR + PconNoHindM8::ryhB::Asp terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    M8::ryhB leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::sodB leader

    Expression

    Bacterial

    Mutation

    Promoter

    pConShortNoHind and pLtetO-1

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pHL2084

    Plasmid

    #62839

    Purpose

    Lim Lab plasmid: AmpR + PconShortNoHindM8::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1

    Depositor

    Han Lim

    Article

    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

    Inserts

    m8::glmZ leader
    T710 hfq leader
    gfp
    

    Use

    Synthetic Biology

    Tags

    m9::glmS

    Expression

    Bacterial

    Mutation

    Promoter

    pConShortNoHind, pLlacO-1, and pLtetO-1

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

17. PA-mCherry-miniSOG-CAF1-C-10

    Plasmid

    #57784

    Purpose

    Localization: Chromatin Assembly Factor 1, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    CAF1
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PA-mCherry-miniSOG-C-Src-7

    Plasmid

    #57785

    Purpose

    Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    C-Src
    

    Use

    Tags

    PA-mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. miniSOG-VASP-5

    Plasmid

    #57778

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    VASP
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. miniSOG-Talin-C-10

    Plasmid

    #57776

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Talin
    

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

21. tdminiSOG-RatLC3

    Plasmid

    #57807

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    LC3
    

    Use

    Tags

    tdminiSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. mCherry-miniSOG-Talin-N-10

    Plasmid

    #55096

    Purpose

    Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Talin
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

23. RFN_EGFP_84

    Plasmid

    #60076

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

24. RFN_EGFP_83

    Plasmid

    #60075

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

25. RFN_EGFP_82

    Plasmid

    #60074

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

26. RFN_EGFP_80

    Plasmid

    #60072

    Purpose

    Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    pair of multiplex gRNAs targeting EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

27. mCherry-miniSOG-Profilin-10

    Plasmid

    #55093

    Purpose

    Localization: Cytoskeleton, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Insert

    Profilin
    

    Use

    Tags

    mCherry-miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

28. TALEN-CCR5R18-NtermN3

    Plasmid

    #51449

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N3 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q, K153Q, and R154Q

    Promoter

    CMV

    Available since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmalc4x_G252R_TEV

    Plasmid

    #47704

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with G252R mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Glycine 252 Arginine

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pmalc4x_K398R_TEV

    Plasmid

    #47701

    Purpose

    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R

    Depositor

    Raquel Lieberman

    Article

    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

    Insert

    Olfactomedin-like domain with K398R mutation (MYOC Human)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    changed Lysine 398 to Arginine

    Promoter

    Ptac

    Available since

    Sept. 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge