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GFP Cav2.2 DomI + DomII pMT2

Plasmid

#206095

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel with a N-terminal GFP tag

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Ad MLP/TPL/SV40

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SUMO-A'-l2-A'-IRES-EmGFP

Plasmid

#209125

Purpose

Lentiviral vector including gene for CC-GEMS ligand SUMO-A'-l2-A' and EmGFP

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'-l2-A'-IRES-EmGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_V222A RAD23B

Plasmid

#201580

Purpose

Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation V222A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_F400A RAD23B

Plasmid

#201581

Purpose

Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains mutation F400A

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-420 POLI

Plasmid

#201582

Purpose

Expresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-420 of DNA polymerase iota

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_aa 1-449 POLH

Plasmid

#201583

Purpose

Expresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

POLH (POLH Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Expresses amino acids 1-449 of DNA polymerase eta

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT POLI

Plasmid

#201440

Purpose

Expresses human DNA polymerase iota with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pJM919_WT His POLI

Plasmid

#201444

Purpose

Low copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA polymerase iota (POLI Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Coding sequence has been optimised for expression…

Promoter

lacIq

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11A(E186Q)-HA

Plasmid

#209261

Purpose

Mammalian expression of ATP11A mutant E186Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11A (ATP11A Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E186Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B4(E170Q)-HA

Plasmid

#209255

Purpose

Mammalian expression of ATP8B4 mutant E170Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E170Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B4(E170Q)-HA

Plasmid

#209243

Purpose

Mammalian expression of ATP8B4 mutant E170Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B4 (ATP8B4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E170Q

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8B1-HA

Plasmid

#209226

Purpose

Mammalian expression of ATP8B1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B1 (ATP8B1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP11C(E184Q)-HA

Plasmid

#209263

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E184Q

Promoter

Available since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8A1(E191Q)-HA

Plasmid

#209252

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E191Q

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP11C(E184Q)-HA

Plasmid

#209251

Purpose

Mammalian expression of ATP11C mutant E184Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E184Q

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B2(E171Q)-HA

Plasmid

#209242

Purpose

Mammalian expression of ATP8B2 mutant E171Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8B2 (ATP8B2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E171Q

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8A1(E191Q)-HA

Plasmid

#209240

Purpose

Mammalian expression of ATP8A1 mutant E191Q

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E191Q

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMx/ATP8A1-HA

Plasmid

#209225

Purpose

Mammalian expression of ATP8A1

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP8A1 (ATP8A1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCA22-SEC61B 5'HA-TriTag (mTagBFP)-3'HA (HDR donor)

Plasmid

#199453

Purpose

CRISPR donor plasmid to insert TriTag (mTagBFP) into the C-terminus of human SEC61B gene

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

HDR donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-PAL1D

Plasmid

#196686

Purpose

Rep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Tags

Expression

Mutation

PTQGTFR insert between amino acids 588 and 589 of…

Promoter

p41

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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