We narrowed to 22,907 results for: Arc

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LBJJ382

Plasmid

#117518

Purpose

Also known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminator

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI

Use

CRISPR

Expression

Plant

Promoter

AtU6-26

Available Since

Oct. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJYDNgp

Plasmid

#162453

Purpose

Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-2Glinker-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

HA nad myc and eUnaG2 fluorescent protein

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHelper_TetR/pTet-Tnpase (carbR)

Plasmid

#213911

Purpose

ATc-inducible himar1C9 transposase expression - carb/amp resistance

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

TetR/ptet ATc-inducible himar1C9 transposase

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMyc-CMV1

Plasmid

#53582

Purpose

mammalian expression vector with Myc tag

Depositor

Linda Yu

Article

Kuo et al Cell Death Differ. 2015 Jan 30. doi: 10.

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6-mTFP1-KanMX6

Plasmid

#69851

Purpose

C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

Depositor

Liza Pon

Article

Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

Insert

mTFP1

Expression

Yeast

Available Since

April 16, 2018

Availability

Academic Institutions and Nonprofits only

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prNFL-EGFP

Plasmid

#132605

Purpose

Expresses EGFP-tagged rat NFL

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGL2_168

Plasmid

#199100

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_169

Plasmid

#199101

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_173

Plasmid

#199105

Purpose

Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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LMAPCW

Plasmid

#205655

Purpose

shRNAmir backbone for RNA interference, with mCherry reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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hSDH5_G78R

Plasmid

#113047

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_G78R (SDHAF2 Human)

Expression

Bacterial

Mutation

G78R, Insert begins at amino acid 39

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMD18-T-pyrF

Plasmid

#174388

Purpose

modified pMD18-T containing pyrF and its native promoter, Ampr

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF

Use

Template for cloning fragments for chromosomal in…

Expression

Bacterial

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pOGG011

Plasmid

#113985

Purpose

Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

pBBR1

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pSH9

Plasmid

#90328

Purpose

Expresses the set-2.a cDNA isoform in the C. elegans intestine

Depositor

Anne Brunet

Article

Han et al Nature. 2017 Apr 13;544(7649):185-190. d

Insert

Pges-1::set-2a

Expression

Worm

Promoter

ges-1

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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[GG][Pin]

Plasmid

#97191

Purpose

Pin nuclease domain with Pum cloning site

Depositor

Edward Boyden

Article

Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

Insert

Pin domain

Use

Synthetic Biology

Tags

Pum cloning site

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔRRM1+2

Plasmid

#35267

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

Deletion of residues 298-429

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
p3E-P2A-CFP no-pA

Plasmid

#80827

Purpose

P2A followed by CFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-CFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Myc-PSFΔN

Plasmid

#35184

Depositor

Benjamin Blencowe

Article

Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

Insert

PSF (SFPQ Human)

Tags

Myc

Expression

Mammalian

Mutation

N-terminal deletion of residues 9-264

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
A0A075HNX4

Plasmid

#177695

Purpose

expresses a fatty alcohol oxidase in E. coli

Depositor

Martin Engqvist

Article

Rembeza et al Chembiochem. 2022 Jan 19;23(2):e20210051

Insert

Long chain alcohol oxidase

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet26

Plasmid

#107448

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met26 (met26 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet16

Plasmid

#107452

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met16 (met16 Fission Yeast)

Tags

V5-His

Expression

Yeast

Promoter

GAL1

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEpic

Plasmid

#84372

Purpose

3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry_LD 0_TEV

Plasmid

#58868

Purpose

MESA protease chain with mCherry ectodomain and no linker domain

Depositor

Joshua Leonard

Article

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA protease chain with mCherry ectodomain and TEV protease

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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TRE.IkBSR-Flag Pgk.Cre

Plasmid

#22504

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

IkBSR

Use

Cre/Lox and Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG5

Plasmid

#193805

Purpose

Protein production of ChemoG5 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG5

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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LMAPPW

Plasmid

#205657

Purpose

shRNAmir backbone for RNA interference, with Puromycin selection

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

mCMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAct::Raf

Plasmid

#11341

Depositor

Michael Wigler

Article

Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

Insert

byr-2 (byr2 Fission Yeast)

Tags

Gal4 activation domain

Expression

Yeast

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
CDS_mALS

Plasmid

#136081

Purpose

Confers plant resistance to chlorsulfuron

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_mALS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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