We narrowed to 7,672 results for: CCH

1. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Expression

   Bacterial

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-hAtg12

   Plasmid

   #87869

   Purpose

   Express a EGFP-human Atg12/Apg12 in mammalian cells

   Depositor

   Eiki Kominami, Isei Tanida

   Article

   Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

   Insert

   ATG12 (ATG12 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST47-MCU-GFP

   Plasmid

   #31732

   Depositor

   Vamsi Mootha

   Article

   Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

   Insert

   MCU (MCU Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available Since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

   Plasmid

   #158585

   Purpose

   Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   membrane localization signal-tagBFP-wrmScarlet11
   

   Tags

   tagBFP

   Expression

   Yeast

   Mutation

   GGGS linker

   Promoter

   TEF

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

   Plasmid

   #89874

   Purpose

   expresses DRD2-iTango component in mammalian cells for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   DRD2-V2tail-TevN-BLITz1-TetR-VP16
   

   Use

   AAV

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   short isoform of DRD2 (deletion of 242-270)

   Promoter

   Human synapsin

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAL005

   Plasmid

   #233865

   Purpose

   MoClo-YTK URA multigene plasmid with MCS; ABA systems; pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2

   Depositor

   Jeff Hasty

   Article

   O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

   Insert

   pTDH3-PhlF-NES-PYL1-tADH1; pTDH3-NLS-VP48-2xOaf1-ABI-tENO1; phlfO7-pLEU2m-MCS-tENO2
   

   Expression

   Yeast

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGFP-HBO1-G485A pc2201

   Plasmid

   #167570

   Purpose

   The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

   Depositor

   Cristina Cardoso

   Article

   Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

   Insert

   histone acetyltransferase 1 (KAT7 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G485A

   Promoter

   CMV

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

   Plasmid

   #85038

   Purpose

   For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   FRT-stop-FRT
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nef-CIAO2-Flp

   Plasmid

   #149296

   Purpose

   Cre-dependent expression of Flp recombinase.

   Depositor

   Edward Callaway

   Article

   Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

   Insert

   Flp recombinase (flp Budding Yeast)
   

   Use

   AAV

   Mutation

   Derived from the mouse codon-optomized FlpO. The…

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-4493NES

    Plasmid

    #138373

    Purpose

    Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-hGABARAP

    Plasmid

    #87871

    Purpose

    Express a EGFP-human GABARAP in mammalian cells

    Depositor

    Eiki Kominami, Isei Tanida

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    GABARAP (GABARAP Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-nef-CIAO-Flp

    Plasmid

    #149294

    Purpose

    Cre-dependent expression of Flp recombinase.

    Depositor

    Edward Callaway

    Article

    Fischer et al Proc Natl Acad Sci U S A. 2019 Dec 16. p

    Insert

    Flp recombinase (flp Budding Yeast)
    

    Use

    AAV

    Mutation

    Derived from the mouse codon-optomized FlpO. The…

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMVS102_P3-GFP-NATMX6

    Plasmid

    #99048

    Purpose

    EGFP reporter with six binding sites for the Zif268 DNA binding domain

    Depositor

    Barak Cohen

    Article

    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

    Inserts

    McIsaac 2014 P3 promoter
    eGFP
    clonNAT resistance
    

    Expression

    Yeast

    Mutation

    GAL1 promoter with 4 GAL4 sites removed and repla…

    Promoter

    McIsaac 2014 P3 promoter

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mcherry p62 delta UBA silent

    Plasmid

    #187984

    Purpose

    mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking UBA domain (aa389-434) but it has the sil…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-mCherry-mCherry-FLARE-AKAR

    Plasmid

    #123333

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-AKAR
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mcherry-p62 delta PB1 silent

    Plasmid

    #187985

    Purpose

    human p62 lacking PB1 domain (=aa1-102) with a silent mutation to get resistance for sip62 #5 (Dharmacon). Internal Reference: SMC552

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacking PB1 domain (aa1-102) with a silent mutati…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223-MCU-D261A

    Plasmid

    #31729

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed D261 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9-EcRT-GAL-Kan

    Plasmid

    #232092

    Purpose

    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    SpCas9 and Ec86 reverse transcriptase
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Ec86 reverse transcriptase is codon optimized for…

    Promoter

    GAL1-GAL10

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-MCU-E264A

    Plasmid

    #31730

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E264 to Alanine

    Promoter

    None

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR223-MCU-E257A

    Plasmid

    #31727

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Use

    Pdonr gateway

    Tags

    None

    Mutation

    Changed E257 to Alanine

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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