We narrowed to 9,391 results for: BLI

1. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

2. Hs.KRAS4b G12S

   Plasmid

   #83144

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12S

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG426-GAL-Alpha-synuclein-E46K

   Plasmid

   #181723

   Purpose

   Galactose inducible expression of Alpha-synuclein-E46K

   Depositor

   Alejandro Chavez

   Insert

   Alpha-synuclein (SNCA Human)
   

   Expression

   Yeast

   Mutation

   E46K

   Available Since

   July 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF1-mAtf4-HT-puro

   Plasmid

   #101795

   Purpose

   Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

   Depositor

   Seiichi Oyadomari

   Insert

   Activating transcription factor 4 (Atf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Halo tag

   Expression

   Mammalian

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hs.NRAS G12V

   Plasmid

   #83174

   Purpose

   Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

   Depositor

   Dominic Esposito

   Insert

   neuroblastoma RAS viral oncogene homolog (NRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12V

   Available Since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. JMC044-B12

   Plasmid

   #58752

   Purpose

   bacterial expression of PDB 4NW3

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4NW3 (KMT2A Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available Since

   Aug. 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. ALDH18A1

   Plasmid

   #38947

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. MS2_SC2_S_Delta

   Plasmid

   #175950

   Purpose

   Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

   Depositor

   Paul Freemont

   Inserts

   Maturation Protein
   Coat Protein Dimer
   S gene (S SARS-CoV-2)
   

   Expression

   Bacterial

   Mutation

   Delta (Amino Acid 319-869)

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. PTPN11 in pENTR

   Plasmid

   #216604

   Purpose

   gateway cloning donor vector containing PTPN11

   Depositor

   Douglas Allan, Christopher Loewen

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid

    #230055

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid

    #192838

    Purpose

    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    Tet

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAMK2G

    Plasmid

    #38894

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2G (CAMK2G Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pRSET-His-APEX2

    Plasmid

    #201471

    Purpose

    Recombinant soluble APEX2 for SLAPSHOT labeling

    Depositor

    Yuh-Nung Jan

    Article

    Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

    Insert

    APEX2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV2-CAGIG-LAMP1-mGL

    Plasmid

    #222394

    Purpose

    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

    Depositor

    Pantelis Tsoulfas

    Insert

    LAMP1 and mGreenlantern (LAMP1 Synthetic, Human, Homo Sapiens)
    

    Use

    AAV

    Tags

    LAMP1 and mGreenLantern

    Expression

    Mammalian

    Promoter

    CAG or CAGIG

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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