We narrowed to 15,731 results for: LEA

1. pLVH-EF1a-GFP-P2A-KLF4

   Plasmid

   #130694

   Purpose

   Lentiviral plasmids encoding human KLF4 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKH3-TRIM28-C65A/C68A

   Plasmid

   #92199

   Purpose

   Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   Tif1b (TRIM28 Human, Synthetic)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Cysteines 65 and 68 to Alanines.

   Available Since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-DroshaS300/302A

   Plasmid

   #62530

   Purpose

   Serines 300 and 302 of Drosha are mutated to alanines

   Depositor

   Bharat Ramratnam

   Article

   Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

   Insert

   human Drosha with serines 300 and 302 changed alanines (DROSHA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed serines 300 and 302 to alanines

   Available Since

   March 20, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pB-Tet-On-Arl13b-RhoA sensor

   Plasmid

   #189613

   Purpose

   Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

   Depositor

   David Clapham

   Article

   Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

   Insert

   Arl13b
   

   Tags

   GFP and mScarlet

   Expression

   Mammalian

   Promoter

   Tet3G

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

   Plasmid

   #133025

   Purpose

   Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Monoamine oxidase type A (MAOA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-488

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK092

   Plasmid

   #183146

   Purpose

   pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

   Depositor

   Daan Swarts

   Article

   Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

   Insert

   CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-GFP-EphrinB3-Flag

   Plasmid

   #155012

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis-LMW_FGF2

   Plasmid

   #157657

   Purpose

   Expresses FGF2 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changed Cystein 211,229 to serine, deleted amino …

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO_Ollas.S.V5_mCherry-NLS

   Plasmid

   #178211

   Purpose

   Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

   Depositor

   Brian Brown

   Article

   Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

   Insert

   nPC Tagged mCherry-NLS
   

   Use

   Lentiviral

   Tags

   Nuclear Localization Signal and Ollas.S.V5

   Promoter

   eF1a

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 3xNLS-NLP-cMyc-cMyc LbaCas12a

    Plasmid

    #182125

    Purpose

    pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    3xNLS-NLP-cMyc-cMyc LbaCas12a
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TVBB C-term-mNeongreen

    Plasmid

    #169227

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-3xFLAG-YAP1-S127A

    Plasmid

    #42239

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Gateway

    Tags

    3xFLAG

    Mutation

    Serine 127 changed to alanine

    Promoter

    none

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_UdgX-APOBEC1-UdgX-NG-nCas9

    Plasmid

    #163562

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-APOBEC1-UdgX-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAR100

    Plasmid

    #180377

    Purpose

    pAR100 allows cloning of TCRα and TCRβ genes using restriction enzymes (AflII, ApaI, and SacII).

    Depositor

    Alec Redwood

    Article

    Sooda et al Commun Biol. 2022 Feb 16;5(1):133. doi:

    Inserts

    NFAT-Luciferase
    hCD8A
    

    Expression

    Bacterial and Mammalian

    Promoter

    hEF1-HTLV and minimal promoter and an NFAT respon…

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Syn1-GRAB-HTR6-cilia

    Plasmid

    #189615

    Purpose

    Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    HTR6_GRAB
    

    Use

    AAV

    Promoter

    Syn1

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_UdgX-Anc689-UdgX-NG-nCas9-RBMX

    Plasmid

    #163554

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-Anc689-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSynapsin_NLS_GFP-P2A_SNAP_GluA2

    Plasmid

    #170455

    Purpose

    Expresses SNAP tagged GluA2 in neuronal cells

    Depositor

    Shigeki Watanabe

    Article

    Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

    Insert

    GluA2 (Gria2 Mouse)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINDUCER-21-RUNX1-P2A-ERG

    Plasmid

    #97045

    Purpose

    Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

    Depositor

    George Daley

    Article

    Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

    Insert

    Runt Related Transcription Factor 1, ETS-Related Gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Tet on

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP2440

    Plasmid

    #72250

    Purpose

    Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

    Promoter

    CMV

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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