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pBOB-3xFlag-mGpr75-L144P

Plasmid

#236745

Purpose

Express 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Gpr75 (Gpr75 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

L144P

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-SpyCatcher003

Plasmid

#234545

Purpose

to express SpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-SC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-mGFAP(ABC1D)-ECS-dSpyCatcher003

Plasmid

#234546

Purpose

to express dSpyCatcher on the extracellular side of astrocytic plasmamembrane

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

ECS-dSC

Use

AAV

Mutation

None

Promoter

mGFAP(ABC1D)

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINF AtMKK5

Plasmid

#228396

Purpose

Recombinant expression of His6-tagged Arabidopsis thaliana MKK5 in E. coli

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

MKK5 (MKK5 Mustard Weed)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR4 HopU1

Plasmid

#228395

Purpose

pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

HopU1

Use

Gateway-compatible entry vector

Mutation

Alanine inserted after start Methionine due to Nc…

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-rtTA-T2A-mTagBFP2

Plasmid

#229797

Purpose

Bxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC12-pA

Plasmid

#220764

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al Nat Commun. 2025 Jul 1;16(1):5557. doi:

Insert

Sun1-GFP-WPRE-BC12

Use

AAV

Promoter

CAG

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TAK4

Plasmid

#227094

Purpose

Luciferase reporter vector with MMTV 5' Element cloned downstream of luciferase gene in pHMRΔeluc

Depositor

Farah Mustafa

Article

Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

Insert

MMTV insert spanning from R region to 400 bp of Gag

Use

Luciferase

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET30-10His-FKBP(f36v)-SNAP

Plasmid

#200282

Purpose

For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

FKBP-SNAPtag

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger2

Plasmid

#217266

Purpose

Donor plasmid of Tigger2 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger2 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-MarsTigger8

Plasmid

#217271

Purpose

Donor plasmid of MarsTigger8 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MarsTigger8 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger4

Plasmid

#217264

Purpose

Donor plasmid of Tigger4 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger4 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger3

Plasmid

#217258

Purpose

Donor plasmid of Tigger3 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger3 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger1

Plasmid

#217247

Purpose

Donor plasmid of Tigger1 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger1 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Zaphod2

Plasmid

#217286

Purpose

Donor plasmid of Zaphod2 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Zaphod2 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Zaphod3

Plasmid

#217281

Purpose

Donor plasmid of Zaphod3 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Zaphod3 transposon

Expression

Mammalian

Available Since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Joey1

Plasmid

#217282

Purpose

Donor plasmid of Joey1 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Joey1 transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Zaphod

Plasmid

#217283

Purpose

Donor plasmid of Zaphod Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Zaphod transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-MARWOLEN1

Plasmid

#217285

Purpose

Donor plasmid of MARWOLEN1 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of MARWOLEN1 transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tigger5

Plasmid

#217272

Purpose

Donor plasmid of Tigger5 Transposon

Depositor

Yong E Zhang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of Tigger5 transposon

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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