159,258 results

1. pLV-UbC-Tet1c-dCas9

   Plasmid

   #232178

   Purpose

   Lentiviral cassette that expresses Tet1c-dCas9 fusion with a short linker in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   Tet1 catalytic domain - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN251

   Plasmid

   #188471

   Purpose

   Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   enCas12a-HF (gift from Dr. Keith Joung)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP12014-pAAV-AiE2004m-minCMV-SYFP2-WPRE3-BGHpA (Alias: CN2014)

   Plasmid

   #164457

   Purpose

   AiE2004m is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minCMV

   Available since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Kl-EGFP

   Plasmid

   #45532

   Depositor

   Zipora Yablonka-Reuveni

   Article

   Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

   Insert

   alpha Klotho (Kl Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. RAPpGEX

   Plasmid

   #162241

   Purpose

   Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

   Depositor

   Andreas Faissner

   Article

   Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

   Insert

   lrpap1 (Lrpap1 Mouse)
   

   Use

   Tags

   GST-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-ERBB4

   Plasmid

   #29527

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-S5E2-Gq-P2A-dTomato

   Plasmid

   #135635

   Purpose

   AAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element

   Depositor

   Jordane Dimidschstein

   Article

   Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

   Has Service

   AAV PHP.eB, AAV1, and AAV9

   Insert

   Gq-P2A-dTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   Available since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PM PKA Reporter

   Plasmid

   #104551

   Purpose

   Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Lyn-mTurquoise2-VASP 148 - 164
   

   Use

   Tags

   mTurquoise 2, HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-N-Blast-3xFLAG

   Plasmid

   #207684

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLVX-IRES-Gβγ-BERKY3

    Plasmid

    #158426

    Purpose

    BRET biosensor for detection of free Gβγ in lentiviral vector pLVX-IRES-Hyg

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK-ATF3

    Plasmid

    #26115

    Depositor

    Yihong Ye

    Article

    Wang et al Proc Natl Acad Sci U S A. 2009 Feb 17. 1

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

    Plasmid

    #209079

    Purpose

    Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    sfGFP
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE4G

    Available since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-BAZ2A

    Plasmid

    #65373

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ2A (BAZ2A Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEYFP-N1-Pink1

    Plasmid

    #101874

    Purpose

    Transient expression of Pink1-YFP in mammalian cells

    Depositor

    Richard Youle

    Article

    Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

    Insert

    PTEN induced putative kinase 1 (PINK1 Human)
    

    Use

    Tags

    fluorescent tag EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB415

    Plasmid

    #74809

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF_dCas9

    Plasmid

    #68416

    Purpose

    Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Wnt5A

    Plasmid

    #35911

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt5A (WNT5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MaLionR

    Plasmid

    #113908

    Purpose

    Red ATP sensor

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

    Insert

    MaLionR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge