165,652 results

1. pCDNA3.1-H-Luc_cyto

   Plasmid

   #113929

   Purpose

   Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

   Depositor

   Kai Johnsson

   Article

   Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

   Insert

   H-Luc
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINE GFP nanobody

   Plasmid

   #49172

   Purpose

   Bacterial expression of GFP nano body

   Depositor

   Brett Collins

   Article

   Kubala et al Protein Sci. 2010 Dec;19(12):2389-401. d

   Insert

   GFP nanobody
   

   Tags

   HHHHHH

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP

   Plasmid

   #85428

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-12
   

   Use

   Lentiviral

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEXSY-hyg2.1_LtIFT43_Flag

   Plasmid

   #194433

   Purpose

   Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

   Depositor

   Alan Brown

   Article

   Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

   Insert

   Intraflagellar transport complex subunit 43
   

   Use

   Leishmania tarentolae expression

   Tags

   3X Flag and Linker

   Promoter

   L.donovani promoter elements

   Available Since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Flag-IDH1

   Plasmid

   #62906

   Purpose

   Expresses wildtype IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C3-Sec15

   Plasmid

   #53760

   Purpose

   pEGFP-C3 plasmid that expresses Sec15

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec15 (EXOC6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pInducer20-Blast

   Plasmid

   #109334

   Purpose

   pInducer20 with added blasticidin resistance

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Type

   Empty backbone

   Use

   Lentiviral; Inducible expression

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Active Wnt10B-V5

    Plasmid

    #43823

    Depositor

    Xi He

    Article

    MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

    Insert

    WNT10B (WNT10B Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGD-GmR

    Plasmid

    #74106

    Purpose

    Plasmid containing gentamicin resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    aacC1
    

    Use

    Bacterial vector based on pmb1 replicon

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX304 CMV OMM-FKBP-V5-sTurboID (N)

    Plasmid

    #153006

    Purpose

    Expresses split-TurboID N-terminal fragment targeted to outer mitochondrial membrane

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    OMM (Tom20)-sTurboID (N) (L73)
    

    Use

    Lentiviral

    Tags

    FKBP and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 c-SRC (WT)

    Plasmid

    #42202

    Depositor

    Robert Lefkowitz

    Article

    Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

    Insert

    c-SRC (SRC Human)
    

    Expression

    Mammalian

    Mutation

    L176F mutation compared to reference sequence NM_…

    Promoter

    CMV

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CAG.LSL.EGFP

    Plasmid

    #100047

    Purpose

    AAV mediated Cre-dependent expression of EGFP (Lox-Stop-Lox)

    Depositor

    Hongkui Zeng

    Article

    Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ccdB-Nanoluc

    Plasmid

    #87067

    Purpose

    Mammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Tags

    Nanoluc luciferase

    Expression

    Mammalian

    Available Since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pY016 (pcDNA3.1-hLbCpf1)

    Plasmid

    #69988

    Purpose

    Expresses humanized LbCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223-ERBB3

    Plasmid

    #23874

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ERBB3 (ERBB3 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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