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eBFP2 (eBFP2)_CD

Plasmid

#66034

Purpose

MoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Fluorescent reporter - BFP

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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PB_tre_Cas9

Plasmid

#126029

Purpose

PiggyBac cargo vector expressing doxycycline inducible Cas9

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Inserts

humanized S. pyrogenes Cas9
Hygromycin resistance

Use

CRISPR; Piggybac

Expression

Mammalian

Promoter

EF1 alpha and TRE

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_NPY1.0

Plasmid

#208675

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA_Cloning Vector

Plasmid

#41824

Purpose

An empty gRNA expression vector, used to create a gRNA to a specific sequence.

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Tags

AflII site for Gibson Assembly

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe:GFP

Plasmid

#13788

Purpose

Mammalian expression of enhanced form of Flp recombinase fused to GFP

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe-GFP fusion protein

Use

Flp/frt

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pWZL-Neo-Myr-Flag-DEST

Plasmid

#15300

Purpose

a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

Depositor

Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Promoter

hEF1a

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA c-Jun

Plasmid

#237333

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

Insert

c-Jun (JUN Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB553

Plasmid

#74883

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCW4

Plasmid

#8484

Depositor

Nancy Craig

Article

Waddell et al Genes Dev 1988 Feb;2(2):137-49.

Insert

Tn7ABCDE (tnsA Bacteria)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-SYP-HRP

Plasmid

#117185

Purpose

Plasmid name in publication: pAAV-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

SYP-HRP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCE059-SiT-Cas12a-[Activ]

Plasmid

#128136

Purpose

Express both AsCas12a fused to [Activ] activator domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Activ]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMIIS928

Plasmid

#238211

Purpose

Expresses TaTasR with an N-terminal HA-NLS tag under CMV promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Tags

HA-NLS

Expression

Mammalian

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9-IBM1 (D30)

Plasmid

#239492

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-XTEN16-BFP-IBM1

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCLIP-tag (m) Vector

Plasmid

#101136

Purpose

Expression of protein of interest with CLIP-Tag in Mammalian cells

Depositor

New England Biolabs, Ana Egana

Type

Empty backbone

Tags

CLIP-tag (CLIP10)

Expression

Mammalian

Available Since

Sept. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLENTI-CMV-GFP-TREX1 D18N BLAST

Plasmid

#164225

Purpose

lentiviral plasmid for GFP-hTREX1 D18N (catalytically-deficient)

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

D18N

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pMT525

Plasmid

#131392

Purpose

L1RP retrotransposition reporter

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

L1RP GFP-AI

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

Plasmid

#51084

Purpose

Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

Depositor

Jonathan Ting

Has Service

AAV Retrograde and AAV1

Insert

GCaMP6s

Use

AAV

Tags

P2A-nls-dTomato

Expression

Mammalian

Promoter

human Synapsin1

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pFL

Plasmid

#110739

Purpose

Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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