We narrowed to 15,731 results for: LEA

1. pRK5-Myc-PSK1-beta

   Plasmid

   #197121

   Purpose

   Expression of MYC-tagged PSK1-beta in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Zihni et al J Biol Chem. 2007 Mar 2;282(9):6484-93.

   Insert

   TAOK2 variant beta (aa1-1049) (TAOK2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Wild Type variant beta

   Promoter

   SV40

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHtrA-TEV-His12

   Plasmid

   #137036

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66500.2 (BB_0104 Borrelia burgdorferi B31)
   

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti4/TO-hEPO

   Plasmid

   #50437

   Purpose

   express human erythropoietin under the control of tetracycline-regulatable CMV promoter using lentiviral delivery system

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Blood. 2011 Nov 17;118(20):5420-8. doi:

   Insert

   EPO (EPO Human)
   

   Use

   Lentiviral; Tetracycline-regulatable (t-rex)

   Expression

   Mammalian

   Promoter

   CMV/TO

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pEN017-SpCas9-hiNLS-t-M4

   Plasmid

   #234455

   Purpose

   Expresses hiNLS variant t-M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEN019-SpCas9-hiNLS-t-M1M4

   Plasmid

   #234456

   Purpose

   Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

   Depositor

   Ross Wilson

   Article

   Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

   Insert

   SpCas9 hiNLS t-M1M4
   

   Use

   CRISPR

   Tags

   His6, Nucleoplasmin, SV40, and c-Myc

   Expression

   Bacterial

   Mutation

   Cys at positions 80 and 574 have been mutated to …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-CMV-GCaMP6-210

   Plasmid

   #91778

   Purpose

   Mammalian expression of low affinity calcium-sensing fluorescent protein

   Depositor

   GENIE Project, Douglas Kim

   Article

   de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

   Insert

   GCaMP6-210
   

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. SHMT2-RES

   Plasmid

   #72880

   Purpose

   overexpression

   Depositor

   David Sabatini

   Article

   Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

   Insert

   SHMT2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   silent mutations in site corresponding to shSHMT2…

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

9. EF1a_DIO_mMeCP2-HaloTag

   Plasmid

   #164062

   Purpose

   Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) Under EF1a promoter

   Depositor

   Nathaniel Heintz

   Article

   Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

   Insert

   MeCP2 Isoform 1 (Mecp2 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFPN1-N-gamma-tubulin (334-449)

    Plasmid

    #87859

    Purpose

    Fluorescent fragment of gamma-tubulin containing gamma-tubulin DNA binding domain and NLS

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment from aa 334 to 449

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICSL11060

    Plasmid

    #68264

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for plants (exemplified in Brassica oleracea)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: CsVMV+ CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMIG-hNFATc1/bC

    Plasmid

    #74049

    Purpose

    retroviral expression plasmid for human NFATc1/bC

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human NFATc1, isoform beta-C (NFATC1 Human)
    

    Use

    Retroviral

    Tags

    no tag

    Expression

    Mammalian

    Promoter

    pMSCV-LTRs

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn Con/Foff EYFP

    Plasmid

    #55651

    Purpose

    Cre-on/Flp-off EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    eYFP with introns
    

    Use

    AAV; Cre on/flp off eyfp

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH6-TEV-spTorA-GFP(C)

    Plasmid

    #169041

    Purpose

    GFP preceeded by the signal peptide of TorA (spTorA) with an N-terminal 6xHis tag and TEV cleavage site in pBAD24.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    H6-spTorA-GFP
    

    Tags

    6xHis, TEV cleavage site, and spTorA (signal pept…

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-c-myc-2A-CDK4(R24C)-GFP-Puro

    Plasmid

    #83107

    Purpose

    3rd generation lentiviral expression of C-myc, CDK4(R24C), and GFP separated by 2A self-cleaving peptide

    Depositor

    Andrew Brooks

    Inserts

    c-Myc (MYC Human)
    CDK4 (CDK4 Human)
    eGFP
    

    Use

    Lentiviral

    Tags

    2A self cleaving peptide

    Expression

    Mammalian

    Mutation

    R24C and p64 isoform

    Promoter

    hPGK and hPGK (single transcript)

    Available Since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. TVBB N-term-mScarlet

    Plasmid

    #169218

    Purpose

    Targeting vector backbone to support a knock-in of mScarlet-Linker at the N-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-BLM DM

    Plasmid

    #80071

    Purpose

    Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

    Depositor

    Nathan Ellis

    Article

    Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

    Insert

    BLM (BLM Human)
    

    Tags

    GFP-BLM

    Expression

    Mammalian

    Mutation

    K317R (AAA>AGA) and K331R (AAA>AGA)

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-DHFR F[1,2]-N (TRP1)

    Plasmid

    #177797

    Purpose

    Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

    Depositor

    Christian Landry

    Article

    Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

    Type

    Empty backbone

    Tags

    DHFR F[1,2]

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. ID1-pLVXT

    Plasmid

    #119280

    Purpose

    Doxycycline-inducible mouse ID1 from 806 cells

    Depositor

    Joan Massague

    Article

    Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

    Insert

    Id1 (Id1 Mouse)
    

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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