We narrowed to 7,804 results for: ttl

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attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Publication

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Promoter

tetO

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDON223 Spike L241-L242-A243-

Plasmid

#180070

Purpose

Sars-CoV-2 protein mutation

Depositor

Markku Varjosalo

Publication

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

SARS-CoV-2 Spike L241-L242-A243- (S SARS-CoV-2)

Tags

MAC-tag

Expression

Mammalian

Mutation

Spike L241-L242-A243-

Available since

Jan. 13, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-ZNF197

Plasmid

#101547

Purpose

Donor Vector containing ZNF197 transcription factor, part of the Human TFome Collection

Depositor

George Church

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF197 (ZNF197 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-FOXE1

Plasmid

#101439

Purpose

Donor Vector containing FOXE1 transcription factor, part of the Human TFome Collection

Depositor

George Church

Publication

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

FOXE1 (FOXE1 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 delta787-843

Plasmid

#166821

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Mutation

_787-843

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway Cloning

Tags

Ubiquitin

Mutation

P1214R

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1972 - [1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Plasmid

#159844

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Flour - TagBFP2(NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA1

Plasmid

#158479

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Publication

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

STOP codon

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8_TtNAM-B1

Plasmid

#124575

Purpose

TtNAM-B1 CDS in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Publication

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-B1

Use

Topo gateway cloning vector

Promoter

None

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCR8_TaNAM-A1(1-217)

Plasmid

#124576

Purpose

NAC domain (1-217) of TaNAM-A1 in the Gateway-compatible pCR8 vector.

Depositor

Cristobal Uauy

Publication

Harrington et al BMC Plant Biol. 2019 Sep 18;19(1):407. d

Insert

TaNAM-A1 (1-217)

Use

Topo gateway cloning vector

Promoter

None

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter

Plasmid

#121818

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IX702: pMVP (L3-L2) OLLAS-IRES-eGFP + polyA

Plasmid

#121748

Purpose

pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + IRES2-eGFP + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion and IRES expression of eGFP reporter

Depositor

Christopher Newgard

Publication

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

OLLAS epitope tag-IRES2-eGFP + polyA

Use

Gateway Cloning and Synthetic Biology

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 no-stop

Plasmid

#123211

Purpose

Gateway entry clone encoding human GABARAPL2 lacking stop codon, suitable for C-terminal tagging

Depositor

Robin Ketteler

Publication

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway Cloning

Mutation

Stop codon removed

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-KAT14-1-546

Plasmid

#118377

Purpose

Gateway compatible donor vector with KAT14-1-546 truncation.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

KAT14 (KAT14 Human)

Use

Donor-plasmid

Mutation

Truncation (aa 1-546)

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_ERBB2_p.R897Q

Plasmid

#81714

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Mutation

I655V; R897Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-SpCas9

Plasmid

#242844

Purpose

Entry vector for Cas9 gateway cloning into a PiggyBac plasmid

Depositor

Siyuan Zhang

Publication

Aleksandrovic et al Nat Commun. 2026 Sep 3;17(1):10497. doi:

Insert

Cas9 (cas9 S. pyogenes)

Use

CRISPR, Gateway Cloning, and Synthetic Biology

Tags

Flag

Expression

Bacterial

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR-D-sp6-cfk-kcnh7a

Plasmid

#255311

Purpose

This gene was cloned into the Gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using an In-Fusion or Gateway system.

Depositor

GuangJun Zhang

Publication

Wu et al J Dev Biol. 2026 Jul 13;14(3):32. doi: 1

Insert

kcnh7a (kcnh7b Zebrafish)

Use

Gateway Cloning; In vitro transcription for ribop…

Expression

Mammalian

Promoter

T7 and Sp6

Available since

July 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCR8-CDK12(sgR)

Plasmid

#208346

Purpose

Gateway cloning entry vector with CDK12 with sgRNA resistant silent mutations. Must be recombined into a DEST vector for expression.

Depositor

Peter S Nelson

Publication

Frank et al eLife 2025

Insert

cyclin dependent kinase 12 (CDK12 Human)

Use

Gateway: entry vector

Mutation

Silent mutations in A88, K90, D92, R94, T715, S71…

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR-Flag-Polb(TM)-C

Plasmid

#216775

Purpose

Flag-Polb(TM), C-terminal domain (AA 91-335); TM = L301R/V303R/V306R

Depositor

Robert Sobol

Publication

Ibrahim et al NAR Cancer. 2025 Dec 31;7(4):zcaf057. do

Insert

Flag-Polb (POLB Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal domain (amino acids 91-335); TM = L301…

Available since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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